Mouse anti-Human PGM3 Monoclonal Antibody | anti-PGM3 antibody

PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) (PE)

Cat. Num. AAA25789

• Gene Names: PGM3; AGM1; PAGM; IMD23; PGM 3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PGM3, Antibody; PGM3 (Phosphoacetylglucosamine Mutase, PAGM, Acetylglucosamine Phosphomutase, N-acetylglucosamine-phosphate Mutasem Phosphoglucomutase-3, AGM1, DKFZp434B187) (PE); anti-PGM3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1E2-1B12
• Specificity: Recognizes human PGM3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PGM3 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: 2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-543 from human PGM3 (AAH01258) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

WB (Western Blot)

(Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data

(Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)

IP (Immunoprecipitation)

(Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)

IHC (Immunohistochemistry)

(Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])

WB (Western Blot)

(Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot)

(PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)

WB (Western Blot)

(Western Blot detection against Immunogen (85.36kD).)

Product Categories/Family for anti-PGM3 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12654830
• NCBI GeneID: 5238
• Molecular Weight: 62,941 Da
• NCBI Official Full Name: Homo sapiens phosphoglucomutase 3, mRNA
• NCBI Official Synonym Full Names: phosphoglucomutase 3
• NCBI Official Symbol: PGM3
• NCBI Official Synonym Symbols: AGM1; PAGM; IMD23; PGM 3
• NCBI Protein Information: phosphoacetylglucosamine mutase; N-acetylglucosamine-phosphate mutase 1; acetylglucosamine phosphomutase