Rabbit PHD3 Monoclonal Antibody | anti-PHD3 antibody

PHD3 Rabbit mAb

Cat. Num. AAA269435

• Gene Names: EGLN3; PHD3; HIFPH3; HIFP4H3
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: PHD3, Antibody; PHD3 Rabbit mAb; PHD3; HIFPH3; HIFP4H3; anti-PHD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: R04-3K9
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

• Applicable Applications for anti-PHD3 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human PHD3
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PHD3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)

WB (Western Blot)

(Western blot analysis of PHD3 in rat lung and mouse lung lysates using PHD3 antibody.)

Related Product Information for anti-PHD3 antibody

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif

Product Categories/Family for anti-PHD3 antibody

Primary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 32129515
• NCBI GeneID: 112399
• UniProt Accession #: Q9H6Z9; Q2TA79; Q3B8N4; Q6P1R2
• Molecular Weight: 239
• NCBI Official Full Name: Egl nine homolog 3
• NCBI Official Synonym Full Names: egl-9 family hypoxia-inducible factor 3
• NCBI Official Symbol: EGLN3
• NCBI Official Synonym Symbols: PHD3; HIFPH3; HIFP4H3
• NCBI Protein Information: egl nine homolog 3; HPH-1; HPH-3; HIF-PH3; HIF prolyl hydroxylase 3; HIF-prolyl hydroxylase 3; egl nine-like protein 3 isoform; hypoxia-inducible factor prolyl hydroxylase 3; prolyl hydroxylase domain-containing protein 3
• UniProt Protein Name: Egl nine homolog 3
• UniProt Gene Name: EGLN3
• UniProt Synonym Gene Names: HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; PHD3
• UniProt Entry Name: EGLN3_HUMAN