IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
PIN4 recombinant antibody
PIN4 Recombinant Monoclonal Antibody
Gene Names
PIN4; EPVH; PAR14; PAR17
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity purified
Synonyms
PIN4, Recombinant Antibody; PIN4 Recombinant Monoclonal Antibody; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8)(Parvulin-14)(Par14)(hPar14)(Parvulin-17)(Par17)(hPar17)(Peptidyl-prolyl cis-trans isomerase Pin4)(PPIase Pin4)(Peptidyl-prolyl cis/trans isomerase EPVH)(hEPVH)(Rotamase Pin4); PIN4; PIN4 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
14A7
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for PIN4 recombinant antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human PIN4
Conjugation
Non-conjugated
Research Area
Epigenetics and Nuclear Signaling; Metabolism; Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of MCF-7 cell with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IHC (Immunohiostchemistry)
(IHC image of AAA278999 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image of AAA278999 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysateAll lanes: PIN4 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14, 17, 15 kDaObserved band size: 12-17 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysateAll lanes: PIN4 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14, 17, 15 kDaObserved band size: 12-17 kDa)
Related Product Information for PIN4 recombinant antibody
Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. {ECO:0000269|PubMed:19369196}.; Isoform 2 binds to double-stranded DNA. {ECO:0000269|PubMed:19369196}.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,810 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isoform 2
NCBI Official Synonym Full Names
protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
NCBI Official Symbol
PIN4
NCBI Official Synonym Symbols
EPVH; PAR14; PAR17
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; hEPVH; hPar14; hPar17; PPIase PIN4; parvulin-14; parvulin-17; rotamase PIN4; eukaryotic parvulin homolog; peptidyl-prolyl cis-trans isomerase Pin4; peptidyl-prolyl cis/trans isomerase EPVH
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
UniProt Gene Name
PIN4
UniProt Synonym Gene Names
Par14; hPar14; Par17; hPar17; PPIase Pin4; hEPVH
UniProt Entry Name
PIN4_HUMAN
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Product Notes
The PIN4 pin4 (Catalog #AAA278999) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The PIN4 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIN4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PIN4 pin4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIN4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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