FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279114 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
PITX3 recombinant antibody
PITX3 Recombinant Monoclonal Antibody
Gene Names
PITX3; ASMD; ASOD; PTX3; CTPP4; CTRCT11
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Affinity-chromatography
Synonyms
PITX3, Recombinant Antibody; PITX3 Recombinant Monoclonal Antibody; Pituitary homeobox 3 (Homeobox protein PITX3)(Paired-like homeodomain transcription factor 3); PITX3; PTX3; PITX3 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
28A9
Purity/Purification
Affinity-chromatography
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for PITX3 recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human PITX3
Conjugate
Non-conjugated
Research Area
Epigenetics and Nuclear Signaling; Neuroscience
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279114 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279114 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 with AAA279114 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degrees C. The secondary antibody was Alexa Fluor 520-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 with AAA279114 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degrees C. The secondary antibody was Alexa Fluor 520-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for PITX3 recombinant antibody
The PITX3 recombinant monoclonal antibody is synthesized through in vitro expression systems that involve cloning the DNA sequences of PITX3 antibodies from immunoreactive rabbits. The immunogen used is a synthesized peptide derived from the human PITX3 protein. Following this, the genes encoding the PITX3 antibodies are incorporated into plasmid vectors, and these recombinant plasmid vectors are transfected into host cells to enable antibody expression. Upon expression, the PITX3 recombinant monoclonal antibody undergoes purification through affinity chromatography. Rigorous testing in ELISA, IF, and FC applications confirms the antibody's reactivity with the human PITX3 protein.
The main role of the PITX3 protein is to function as a transcription factor that regulates the development and maintenance of various components of the eye, including the lens, cornea, and ciliary body. Its proper functioning is essential for normal eye development and vision, and mutations in the PITX3 gene can lead to congenital cataracts and other eye-related disorders.
The main role of the PITX3 protein is to function as a transcription factor that regulates the development and maintenance of various components of the eye, including the lens, cornea, and ciliary body. Its proper functioning is essential for normal eye development and vision, and mutations in the PITX3 gene can lead to congenital cataracts and other eye-related disorders.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,832 Da
NCBI Official Full Name
pituitary homeobox 3
NCBI Official Synonym Full Names
paired-like homeodomain 3
NCBI Official Symbol
PITX3
NCBI Official Synonym Symbols
ASMD; ASOD; PTX3; CTPP4; CTRCT11
NCBI Protein Information
pituitary homeobox 3; homeobox protein PITX3
UniProt Protein Name
Pituitary homeobox 3
UniProt Gene Name
PITX3
UniProt Synonym Gene Names
PTX3
UniProt Entry Name
PITX3_HUMAN
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Product Notes
The PITX3 pitx3 (Catalog #AAA279114) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The PITX3 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PITX3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the PITX3 pitx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PITX3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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