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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with PKA 2 beta (regulatory subunit) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit PKA 2 beta Monoclonal Antibody | anti-PRKAR2B antibody
PKA 2 beta (Regulatory Subunit) Antibody
Gene Names
PRKAR2B; PRKAR2; RII-BETA
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
ProA affinity purified
Synonyms
PKA 2 beta, Antibody; PKA 2 beta (Regulatory Subunit) Antibody; AI451071 antibody; AW061005 antibody; cAMP dependent protein kinase type II beta regulatory chain antibody; cAMP dependent protein kinase type II beta regulatory subunit antibody; cAMP-dependent protein kinase type II-beta regulatory subunit antibody; H RG363E19.2 antibody; KAP3_HUMAN antibody; MGC116401 antibody; Pkarb2 antibody; PRKAR 2 antibody; PRKAR2 antibody; PRKAR2B antibody; Protein kinase cAMP dependent regulatory type II beta antibody; RATDNA antibody; RII beta antibody; RII (beta) antibody; RIIbeta antibody; WUGSC:H RG363E19.2 antibody; anti-PRKAR2B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JA11-69
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PRKAR2B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with PKA 2 beta (regulatory subunit) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of N2A cells with PKA 2 beta (regulatory subunit) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PKA 2 beta (regulatory subunit) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- PKA 2 beta (regulatory subunit) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- PKA 2 beta (regulatory subunit) antibody. Counter stained with hematoxylin.)
Related Product Information for anti-PRKAR2B antibody
The second messenger cyclic AMP mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. One of several regulatory subunits, p-PKA IIbeta reg (cAMP-dependent protein kinase type II-beta regulatory subunit), also known as PRKAR2B, is a 418 amino acid protein that is phosphorylated by the activated catalytic chain. p-PKA IIbeta reg knockout mice exhibit diminished white adipose tissue and were protected against diet-induced obesity and fatty livers, as well as markedly reduced leptin mRNA. Also playing a role in the immune response, p-PKA IIbeta reg suppresses CREB transcriptional activity and down-regulates IL-2 production in T-lymphocytes.
Product Categories/Family for anti-PRKAR2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,302 Da
NCBI Official Full Name
cAMP-dependent protein kinase type II-beta regulatory subunit
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, regulatory, type II, beta
NCBI Official Symbol
PRKAR2B
NCBI Official Synonym Symbols
PRKAR2; RII-BETA
NCBI Protein Information
cAMP-dependent protein kinase type II-beta regulatory subunit; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type II-beta regulatory chain
UniProt Protein Name
cAMP-dependent protein kinase type II-beta regulatory subunit
UniProt Gene Name
PRKAR2B
UniProt Entry Name
KAP3_HUMAN
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Product Notes
The PRKAR2B prkar2b (Catalog #AAA312486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKA 2 beta (Regulatory Subunit) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKA 2 beta can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PRKAR2B prkar2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA 2 beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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