Mouse anti-Human PKA C-alpha (PRKACA) Monoclonal Antibody | anti-PRKACA antibody

[KO Validated] PKA C-alpha (PRKACA) Mouse mAb

Cat. Num. AAA282646

• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purification
• Synonyms: PKA C-alpha (PRKACA), Antibody; [KO Validated] PKA C-alpha (PRKACA) Mouse mAb; CAFD1; PKACA; PPNAD4; A); anti-PRKACA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B, Kappa
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-PRKACA antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein of human [KD Validated] PKA C-alpha (PRKACA).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF7 cells using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb (AAA282646) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of BALB-3T3 cells using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb (AAA282646) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of lysates from wild type(WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T(KD) cells, using [KO Validated] PKA C-alpha (PRKACA) Mouse mAb (AAA282646) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)

WB (Western Blot)

(Western blot analysis of various lysates, using [KO Validated] PKA C-alpha (PRKACA) Mouse mAb (AAA282646) at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.)

Related Product Information for anti-PRKACA antibody

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Product Categories/Family for anti-PRKACA antibody

Protein phosphorylation; Cancer; Signal Transduction; G protein signaling; G-Protein-Coupled Receptors Signaling to MAPK Erk; Kinase; Serine threonine kinases; MAPK-Erk Signaling Pathway; Cell Biology Developmental Biology; Apoptosis; Mitochondrial Contro

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721.1
• NCBI GenBank Nucleotide #: NM_002730.3
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• Molecular Weight: Calculated MW: 41kDa; Observed MW: 40kDa
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN