PKC delta Monoclonal Antibody | anti-PKCdelta antibody

PKC delta Rabbit mAb

Cat. Num. AAA314436

• Gene Names: Prkcd; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: PKC delta, Antibody; PKC delta Rabbit mAb; Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e; anti-PKCdelta antibody

• Host: Recombinant Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: S09-8H6
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-PKCdelta antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human PKC delta
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry of PKC delta in paraffin-embedded Human colon cancer tissue using PKC delta Rabbit mAb at dilution 1/100)

WB (Western Blot)

(Western blot detection of PKC delta in K562,C6,3T3 cell lysates using PKC delta Rabbit mAb(1:1000 diluted).Predicted band size:78kDa.Observed band size:78kDa.)

Related Product Information for anti-PKCdelta antibody

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion. Phosphorylates ELAVL1 in response to angiotensin-2 treatment .

Product Categories/Family for anti-PKCdelta antibody

Primary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 6755082
• NCBI GeneID: 18753
• NCBI Accession #: NP_035233.1
• NCBI GenBank Nucleotide #: NM_011103.3
• UniProt Accession #: P28867; Q91V85; Q9Z333
• Molecular Weight: 80,294 Da
• NCBI Official Full Name: protein kinase C delta type
• NCBI Official Synonym Full Names: protein kinase C, delta
• NCBI Official Symbol: Prkcd
• NCBI Official Synonym Symbols: Pkcd; PKC[d]; AI385711; PKCdelta; D14Ertd420e
• NCBI Protein Information: protein kinase C delta type; nPKC-delta; protein kinase C delta variant IX; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C[d]; tyrosine-protein kinase PRKCD
• UniProt Protein Name: Protein kinase C delta type
• UniProt Gene Name: Prkcd
• UniProt Synonym Gene Names: Pkcd; SDK1