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FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse PLCG1 Monoclonal Antibody | anti-PLCG1 antibody
PLCG1 Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Rat, Monkey
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PLCG1, Antibody; PLCG1 Antibody; 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase gamma-1 antibody; Inositoltrisphosphohydrolase antibody; Monophosphatidylinositol phosphodiesterase antibody; NCKAP3 antibody; Phosphatidylinositol phospholipase C antibody; Phosphoinositidase C antibody; Phosphoinositide phospholipase C antibody; Phosphoinositide phospholipase C-gamma-1 antibody; Phospholipase C 148 antibody; Phospholipase C gamma 1 antibody; Phospholipase C-gamma-1 antibody; Phospholipase C-II antibody; PLC gamma 1 antibody; PLC II antibody; PLC-148 antibody; PLC-gamma-1 antibody; PLC-II antibody; PLC1 antibody; PLC148 antibody; Plcg1 antibody; PLCG1_HUMAN antibody; PLCgamma1 antibody; anti-PLCG1 antibody
Host
Mouse
Reactivity
Human, Rat, Monkey
Clonality
Monoclonal
Clone Number
D11-C2
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PLCG1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry)
(ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1, 000 dilution. Positive control Line1: Hela Line2: A431 Line3: C6 Line4: NIH/3T3 Line5: COS7 Line6: HCT116)
WB (Western Blot)
(Western blot analysis of PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1, 000 dilution. Positive control Line1: Hela Line2: A431 Line3: C6 Line4: NIH/3T3 Line5: COS7 Line6: HCT116)
WB (Western Blot)
(Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-PLCG1 antibody
Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases.
Product Categories/Family for anti-PLCG1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
148,532 Da
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
NCBI Official Synonym Full Names
phospholipase C, gamma 1
NCBI Official Symbol
PLCG1
NCBI Official Synonym Symbols
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylin
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
UniProt Gene Name
PLCG1
UniProt Synonym Gene Names
PLC1; PLC-II; PLC-gamma-1
UniProt Entry Name
PLCG1_HUMAN
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Product Notes
The PLCG1 plcg1 (Catalog #AAA29899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PLCG1 Antibody reacts with Human, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PLCG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the PLCG1 plcg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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