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Application Data
Mouse anti-Human, Mouse PMEL/Melanoma gp100 (HMB45) Monoclonal Antibody | anti-PMEL antibody
PMEL/Melanoma gp100 (HMB45)(ABT255) Antibody
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
Purity
Protein G
Synonyms
PMEL/Melanoma gp100 (HMB45); Monoclonal Antibody; PMEL/Melanoma gp100 (HMB45)(ABT255) Antibody; 95 kDa melanocyte specific secreted glycoprotein; 95 kDa melanocyte-specific secreted glycoprotein; D12S53E; gp100; M-beta; ME20; ME20 M/ME20 S; ME20-M; ME20-S; ME20M; ME20M/ME20S; ME20S; Melanocyte lineage specific antigen GP100; Melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocyte protein Pmel 17 precursor; Melanocytes lineage-specific antigen GP100; Melanoma associated ME20 antigen; Melanoma gp100; Melanoma-associated ME20 antigen; Melanosomal matrix protein 17; Melanosomal matrix protein17; P1; p100; p26; PMEL 17; PMEL; PMEL_HUMAN; PMEL17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; SI; SIL; SILV; Silver (mouse homolog) like; Silver homolog; Silver locus protein homolog; Silver; mouse; homolog of; 95 kDa melanocyte specific secreted glycoprotein?95 kDa melanocyte-specific secreted glycoprotein?D12S53E?gp100?M-beta?ME20?ME20 M/ME20 S?ME20-M?ME20-S?ME20M?ME20M/ME20S?ME20S?Melanocyte lineage specific antigen GP100?Melanocyte protein mel 17?Melanocyte protein Pmel 17?Melanocyte protein Pmel 17 precursor?Melanocytes lineage-specific antigen GP100?Melanoma associated ME20 antigen?Melanoma gp100?Melanoma-associated ME20 antigen?Melanosomal matrix protein 17?Melanosomal matrix protein17?P1?p100?p26?PMEL 17?PMEL?PMEL_HUMAN?PMEL17?Premelanosome protein?Secreted melanoma-associated ME20 antigen?SI?SIL?SILV?Silver (Mouse homolog) like?Silver homolog?Silver locus protein homolog?Silver; Mouse; anti-PMEL antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
ABT255
Specificity
The antibody can specifically recognize human PMEL protein.
Purity/Purification
Protein G
Form/Format
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applicable Applications for anti-PMEL antibody
Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:200-1000
WB: 1:500-2000
IF: 1:100-500
ELISA: 1:1000-5000
WB: 1:500-2000
IF: 1:100-500
ELISA: 1:1000-5000
Preparation and Storage
Store at -20 degree C/1 year
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SDS-PAGE
(Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HMB45 (ABT255) antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE
(Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HMB45 (ABT255) antibody. The HRP-conjugated Goat anti-M)
Product Categories/Family for anti-PMEL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,255 Da
NCBI Official Full Name
Melanocyte protein PMEL
NCBI Official Synonym Full Names
premelanosome protein
NCBI Official Symbol
PMEL
NCBI Official Synonym Symbols
P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
NCBI Protein Information
melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100
UniProt Protein Name
Melanocyte protein PMEL
UniProt Gene Name
PMEL
UniProt Synonym Gene Names
D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
UniProt Entry Name
PMEL_HUMAN
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Product Notes
The PMEL pmel (Catalog #AAA30912) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PMEL/Melanoma gp100 (HMB45)(ABT255) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PMEL/Melanoma gp100 (HMB45) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). IHC: 1:200-1000 WB: 1:500-2000 IF: 1:100-500 ELISA: 1:1000-5000. Researchers should empirically determine the suitability of the PMEL pmel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMEL/Melanoma gp100 (HMB45), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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