FCM/FACS (Flow Cytometry)
(FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).)
Mouse anti-Human Podoplanin, PE-conjugated Monoclonal Antibody | anti-PDPN antibody
Mouse Anti-Human Podoplanin
Gene Names
PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein G purified
Synonyms
Podoplanin, PE-conjugated, Antibody; Mouse Anti-Human Podoplanin; Anti-human Podoplanin-PE (#18H5); Glycoprotein 36; GP36; T1-alpha; PA2.26 antigen; anti-PDPN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
18H5 / IgG1
Specificity
The unconjugated monoclonal antibody will detect native human Podoplanin in WB and on the surface of human LEC.
Purity/Purification
Protein G purified
Form/Format
Liquid
Concentration
200 ug/ml (varies by lot)
Sequence Length
120
Applicable Applications for anti-PDPN antibody
FCM/FACS (Flow Cytometry)
Immunogen
MDCK cells stably expressing gp36 (Podoplanin) protein
Conjugation
PE
Preparation
Monoclonal antibodies were produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen.
Buffer/Stabilizer
PBS; 1 % BSA
Preservative
0,02 % sodium azide
Warnings
Reagents contains sodium azide. Under acidic conditions sodium azide yield hydrazoic acid, this is extremely toxic. Acide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive condition may develop.
Preparation and Storage
Store protected from light at 2-8°C. DO NOT FREEZE!
FCM/FACS (Flow Cytometry)
(FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).)
FCM/FACS (Flow Cytometry)
(FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).)
Related Product Information for anti-PDPN antibody
Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
References
1. Breiteneder-Geleff et al, Am J Pathol 154:385, 1999 2. Kerjaschki et al, Nature Med 12:230, 2006 3. Kriehuber et al, J Exp Med 194:797, 2001 4. Zimmer. et al, Biochem J 341:277, 1999 5. Kato et al, Tumour Biol 26:195, 2005 6. Katsue-Inoue et al, J Biol Chem 282:25993, 2007 7. Kato et al, Oncogene 23:8552, 2004 8. Schacht. et al, Am J Pathol 166:913, 2005 9. Mishima et al, Acta Neuropathol 111:483, 2006
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
podoplanin isoform c
NCBI Official Synonym Full Names
podoplanin
NCBI Official Symbol
PDPN
NCBI Official Synonym Symbols
T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
NCBI Protein Information
podoplanin; T1-alpha; hT1alpha-1; hT1alpha-2; PA2.26 antigen; glycoprotein 36; glycoprotein, 36-KD; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2)
UniProt Protein Name
Podoplanin
UniProt Gene Name
PDPN
UniProt Synonym Gene Names
GP36; Gp36; T1A
UniProt Entry Name
PDPN_HUMAN
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Product Notes
The PDPN pdpn (Catalog #AAA79088) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Podoplanin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Podoplanin, PE-conjugated can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PDPN pdpn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Podoplanin, PE-conjugated, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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