FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Human PPIL1 Monoclonal Antibody | anti-PPIL1 antibody
Anti-PPIL1 Antibody, Mouse Monoclonal
Gene Names
PPIL1; CYPL1; hCyPX; PPIase; CGI-124
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Protein A
Synonyms
PPIL1, Antibody; Anti-PPIL1 Antibody, Mouse Monoclonal; PPIL1 Antibody, Mouse MAb; peptidylprolyl isomerase (cyclophilin)-like 1; Anti-CGI-124 Antibody; Anti-CYPL1 Antibody; Anti-hCyPX Antibody; Anti-PPIase Antibody; anti-PPIL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
08
Specificity
Human PPIL1.
No cross-reactivity in ELISA with E Coli cell lysate
No cross-reactivity in ELISA with E Coli cell lysate
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPIL1 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human PPIL1 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPIL1 (rh PPIL1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
IP (Immunoprecipitation)
(PPIL1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)
IP (Immunoprecipitation)
(PPIL1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg U87MG Whole Cell Lysate2 uL anti-PPIL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIL1 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)
WB (Western Blot)
(Anti-PPIL1 mouse monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PPIL1 mouse monoclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PPIL1 antibody
PPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take a significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy.
References
Xu C. et al., 2005, J Biomol NMR. 31 (2): 179-80. Ozaki K. et al., 1997, Cytogenet Cell Genet. 72 (2-3): 242-5. Mann SS. et al., 1999, Cytogenet Cell Genet. 83 (3-4): 228-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,237 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase-like 1
NCBI Official Synonym Full Names
peptidylprolyl isomerase (cyclophilin)-like 1
NCBI Official Symbol
PPIL1
NCBI Official Synonym Symbols
CYPL1; hCyPX; PPIase; CGI-124
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase-like 1; cyclophilin-related gene 1; rotamase PPIL1
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase-like 1
UniProt Gene Name
PPIL1
UniProt Synonym Gene Names
CYPL1; PPIase
UniProt Entry Name
PPIL1_HUMAN
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Product Notes
The PPIL1 ppil1 (Catalog #AAA255724) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPIL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIL1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PPIL1 ppil1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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