IF (Immunofluorescence)
(ICC/IF analysis of PPM1F in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1F antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human PPM1F Monoclonal Antibody | anti-PPM1F antibody
PPM1F antibody
Gene Names
PPM1F; CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
PPM1F, Antibody; PPM1F antibody; Protein phosphatase 1F; CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2; anti-PPM1F antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT6D11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
454
Applicable Applications for anti-PPM1F antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human PPM1F (1-454aa) purified from E. coli
Clone Note
Anti-human PPM1F mAb, clone AT6D11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPM1F protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PPM1F in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1F antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of PPM1F in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1F antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PPM1F Transfected 293T cell lysate)
WB (Western Blot)
(The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PPM1F Transfected 293T cell lysate)
Related Product Information for anti-PPM1F antibody
PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
Product Categories/Family for anti-PPM1F antibody
References
Koh, CG., et al. (2002) Curr Biol 12 (4): 317-21; Tan, KM., et al. (2001) J Biol Chem 276 (47): 44193-202
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,518 Da
NCBI Official Full Name
protein phosphatase 1F
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1F
NCBI Official Symbol
PPM1F
NCBI Official Synonym Symbols
CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
NCBI Protein Information
protein phosphatase 1F
UniProt Protein Name
Protein phosphatase 1F
UniProt Gene Name
PPM1F
UniProt Synonym Gene Names
KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
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Product Notes
The PPM1F ppm1f (Catalog #AAA48123) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPM1F antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1F can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PPM1F ppm1f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1F, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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