IF (Immunofluorescence)
(ICC/IF analysis of PPP3CA in HeLa cells. The cell was stained with ATGA0505 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human PPP3CA Monoclonal Antibody | anti-PPP3CA antibody
PPP3CA
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; CALNA1; IECEE1
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
PPP3CA, Antibody; PPP3CA; Serine/threonine-protein phosphatase 2B catalytic subunit alpha; CALN; CALNA; CALNA1; CCN1; CNA1; PPP2B; anti-PPP3CA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1E11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
511
Applicable Applications for anti-PPP3CA antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human PPP3CA (1-511aa) purified from E. coli
Clone Note
Anti-human PPP3CA mAb, clone AT1E11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP3CA protein.
Preparation and Storage
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PPP3CA in HeLa cells. The cell was stained with ATGA0505 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of PPP3CA in HeLa cells. The cell was stained with ATGA0505 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP3CA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 1.: 293T cell lysate Lane 1.: LNCaP cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP3CA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 1.: 293T cell lysate Lane 1.: LNCaP cell lysate)
Related Product Information for anti-PPP3CA antibody
PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2.
Product Categories/Family for anti-PPP3CA antibody
References
Ostenfeld MS., et al. (2010) Oncogene. 29(7):1073-84. Rodriguez A., et al. (2009) Mol Cell. 33(5):616-26.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,245 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 2
NCBI Official Synonym Full Names
protein phosphatase 3 catalytic subunit alpha
NCBI Official Symbol
PPP3CA
NCBI Official Synonym Symbols
CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; CALNA1; IECEE1
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Gene Name
PPP3CA
UniProt Synonym Gene Names
CALNA; CNA
Similar Products
Product Notes
The PPP3CA ppp3ca (Catalog #AAA48961) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPP3CA reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP3CA can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PPP3CA ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP3CA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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