<
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody (AAA283540). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
Mouse anti-Human, mouse PRDM2 (RIZ1) Monoclonal Antibody | anti-PRDM2 antibody
PRDM2 (RIZ1) Antibody
Gene Names
PRDM2; RIZ; KMT8; RIZ1; RIZ2; MTB-ZF; HUMHOXY1
Reactivity
Human, mouse
Applications
ELISA, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Synonyms
PRDM2 (RIZ1), Antibody; PRDM2 (RIZ1) Antibody; PR domain zinc finger protein 2; GATA-3-binding protein G3B; Lysine N-methyltransferase 8; MTB-ZF; MTE-binding protein; PR domain-containing protein 2; Retinoblastoma protein-interacting zinc finger protein; Zinc finger protein RIZ; PRDM2; KMT8; RIZ; anti-PRDM2 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
33AT1045
Specificity
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).
Purity/Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
1-347
Applicable Applications for anti-PRDM2 antibody
ELISA, WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Function
S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
Cellular Location
Nucleus
Tissue Location
Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen Isoform 1 is expressed in testis at much higher level than isoform 3.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody (AAA283540). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using anti-RIZ1 antibody (AAA283540). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes.(B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 (AAA283540) and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes.(B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with antibody to RIZ1 (AAA283540) and control immunoglobulin G as indicated. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1 (AAA283540). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
ChIP (Chromatin Immunoprecipitation)
(ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with antibody against RIZ1 (AAA283540). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter. Adapted from Fig 6 in Carling et al., 2004 (see citation 2).)
WB (Western Blot)
(Western blot analysis of anti-PRDM2 Mab in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-PRDM2 Mab in lysate from PRDM2 transgenic lysate.Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PRDM2 antibody
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-PRDM2 antibody
References
Genotypes and haplotypes of the estrogen receptor genes, but not the retinoblastoma-interacting zinc finger protein 1 gene, are associated with osteoporosis. HarslÞf T, et al. Calcif Tissue Int, 2010 Jul. PMID 20508921.
DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer. Cheng HY, et al. Eur J Med Res, 2010 Jan 29. PMID 20159667.
Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer. de Vogel S, et al. Cancer Epidemiol Biomarkers Prev, 2009 Nov. PMID 19843671.
Expression of RIZ1 protein (Retinoblastoma-interacting zinc-finger protein 1) in prostate cancer epithelial cells changes with cancer grade progression and is modulated in vitro by DHT and E2. Rossi V, et al. J Cell Physiol, 2009 Dec. PMID 19746436.
RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression. Lakshmikuttyamma A, et al. J Hematol Oncol, 2009 Jul 14. PMID 19602237.
DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer. Cheng HY, et al. Eur J Med Res, 2010 Jan 29. PMID 20159667.
Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer. de Vogel S, et al. Cancer Epidemiol Biomarkers Prev, 2009 Nov. PMID 19843671.
Expression of RIZ1 protein (Retinoblastoma-interacting zinc-finger protein 1) in prostate cancer epithelial cells changes with cancer grade progression and is modulated in vitro by DHT and E2. Rossi V, et al. J Cell Physiol, 2009 Dec. PMID 19746436.
RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression. Lakshmikuttyamma A, et al. J Hematol Oncol, 2009 Jul 14. PMID 19602237.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
PR domain zinc finger protein 2 isoform c
NCBI Official Synonym Full Names
PR domain containing 2, with ZNF domain
NCBI Official Symbol
PRDM2
NCBI Official Synonym Symbols
RIZ; KMT8; RIZ1; RIZ2; MTB-ZF; HUMHOXY1
NCBI Protein Information
PR domain zinc finger protein 2
UniProt Protein Name
PR domain zinc finger protein 2
UniProt Gene Name
PRDM2
UniProt Synonym Gene Names
KMT8; RIZ
UniProt Entry Name
PRDM2_HUMAN
Similar Products
Product Notes
The PRDM2 prdm2 (Catalog #AAA283540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-347. The PRDM2 (RIZ1) Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM2 (RIZ1) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the PRDM2 prdm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM2 (RIZ1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.