IB (Immunoblot)
(Bands of 85kDa and 88kDa are detected.)
Mouse Prekallikrein Heavy Chain Monoclonal Antibody
Prekallikrein Heavy Chain Monoclonal Antibody
Applications
Immunoblot, Western Blot, ELISA
Purity
Purified human prekallikrein.
Synonyms
Prekallikrein Heavy Chain, Antibody; Prekallikrein Heavy Chain Monoclonal Antibody; Anti-Prekallikrein Heavy Chain; Prekallikrein; anti-Prekallikrein Heavy Chain antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
13G11
Specificity
Anti-Prekallikrein Heavy Chain [MurineMAb recognizes the heavy chain of human prekallikrein.]
This antibody specifically recognizes in human plasma two variants (88 kDa and 85 kDa) of prekallikrein and its activation products kallikrein (88 kDa and 85 kDa), the complexes formed by kallikrein with its endogenous inhibitors C1 inhibitor, alpha-2-macroglobulin and antithrombin III, and 45 kDa prekallikrein/kallikrein fragment(s). It also recognizes prekallikrein and its activation products in chimpanzee, rhesus, and baboon plasmas. The epitope for this antibody, located on the prekallikrein/kallikrein heavy chain, is involved in the interaction between prekallikrein and factor XIIa. This antibody inhibits prekallikrein activation in human and rhesus plasmas by approximately 60-80% and 55%,respectively. This antibody does not cross-react with tissue kallikrein.
This antibody specifically recognizes in human plasma two variants (88 kDa and 85 kDa) of prekallikrein and its activation products kallikrein (88 kDa and 85 kDa), the complexes formed by kallikrein with its endogenous inhibitors C1 inhibitor, alpha-2-macroglobulin and antithrombin III, and 45 kDa prekallikrein/kallikrein fragment(s). It also recognizes prekallikrein and its activation products in chimpanzee, rhesus, and baboon plasmas. The epitope for this antibody, located on the prekallikrein/kallikrein heavy chain, is involved in the interaction between prekallikrein and factor XIIa. This antibody inhibits prekallikrein activation in human and rhesus plasmas by approximately 60-80% and 55%,respectively. This antibody does not cross-react with tissue kallikrein.
Purity/Purification
Purified human prekallikrein.
Form/Format
Protein G-purified antibody in PBS, pH 7.4.
Concentration
1.5mg/mL (varies by lot)
Applicable Applications for anti-Prekallikrein Heavy Chain antibody
IB (Immunoblot), ELISA
Preservatives
None
Appearance
Clear Liquid
Preparation and Storage
This antibody is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
IB (Immunoblot)
(Bands of 85kDa and 88kDa are detected.)
IB (Immunoblot)
(Bands of 85kDa and 88kDa are detected.)
Related Product Information for anti-Prekallikrein Heavy Chain antibody
Background: Human prekallikrein, a serine protease zymogen, is present in plasma as two variants, 88kDa and 85kDa, probably due to different carbohydrate content. Approximately 75% of the total prekallikrein in plasma is noncovalently associated with high molecular weight kininogen (HMWK). HMWK mediates binding of prekallikrein to a negatively-charged surface which is required for its optimal activation to kallikrein by activated factor XII. Although kallikrein light and heavy chains can behave as functionally independent domains, the interaction between these two chains is essential for full biologic activity.
Product Categories/Family for anti-Prekallikrein Heavy Chain antibody
References
Veloso et al. (1987) Blood 70: 1053- 1062; Veloso et al. (1989) Adv Exp Med Biol 247: 499-505; Veloso D and Colman RW (1991) Thrombosis and Haemostasis 65: 382-388; Veloso et al. (1992) Thrombosis and Haemostasis 68: 526-533; Veloso D (1996) Immunopharmacology 33: 374-376.
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Product Notes
The Prekallikrein Heavy Chain (Catalog #AAA47712) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Prekallikrein Heavy Chain can be used in a range of immunoassay formats including, but not limited to, IB (Immunoblot), ELISA. Researchers should empirically determine the suitability of the Prekallikrein Heavy Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prekallikrein Heavy Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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