WB (Western Blot)
(PRKAA2 antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).)
Mouse anti-Human PRKAA2 Monoclonal Antibody | anti-PRKAA2 antibody
PRKAA2 Antibody
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Synonyms
PRKAA2, Antibody; PRKAA2 Antibody; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; PRKAA2; AMPK; AMPK2; anti-PRKAA2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,K
Clone Number
206CT5.4.2
Specificity
This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
552
Applicable Applications for anti-PRKAA2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PRKAA2 antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).)
WB (Western Blot)
(PRKAA2 antibody western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with PRKAA2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PRKAA2 immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with PRKAA2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRKAA2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).PRKAA2 immunoreactivity is localized to Cytoplasm significantly.)
Related Product Information for anti-PRKAA2 antibody
The protein encoded by this gene is a catalytic subunit of
the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol.
Studies of the mouse counterpart suggest that this catalytic
subunit may control whole-body insulin sensitivity and is necessary
for maintaining myocardial energy homeostasis during ischemia.
the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol.
Studies of the mouse counterpart suggest that this catalytic
subunit may control whole-body insulin sensitivity and is necessary
for maintaining myocardial energy homeostasis during ischemia.
Product Categories/Family for anti-PRKAA2 antibody
References
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Bungard, D., et al. Science 329(5996):1201-1205(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Bungard, D., et al. Science 329(5996):1201-1205(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62320
NCBI Official Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 2 catalytic subunit
NCBI Official Symbol
PRKAA2
NCBI Official Synonym Symbols
AMPK; AMPK2; PRKAA; AMPKa2
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Gene Name
PRKAA2
UniProt Synonym Gene Names
AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK2_HUMAN
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Product Notes
The PRKAA2 prkaa2 (Catalog #AAA283480) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PRKAA2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAA2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRKAA2 prkaa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAA2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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