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product-image-AAA21219_IHC9.jpg IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: human Lymph node TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Mouse anti-Human, Pig Procollagen III N-Terminal Propeptide (PIIINP) Monoclonal Antibody | anti-PIIINP antibody

Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

Gene Names
COL3A1; EDS4A
Reactivity
Human, Pig
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Procollagen III N-Terminal Propeptide (PIIINP), Antibody; Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP); Procollagen III N-Terminal Propeptide; P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide; anti-PIIINP antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Pig
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
J38-2
Specificity
The antibody is a mouse monoclonal antibody raised against PIIINP. It has been selected for its ability to recognize PIIINP in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Gln24~Pro153
Applicable Applications for anti-PIIINP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen
Recombinant Procollagen III N-Terminal Propeptide (PIIINP)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

IHC (Immunohistchemistry)

(DAB staining on IHC-P;Sample: human Lymph node TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC9.jpg IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: human Lymph node TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Small intestine TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC8.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Small intestine TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Placenta TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC7.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Placenta TissuePrimary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistchemistry)

(DAB staining on IHC-P;Sample: human Uterus Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC6.jpg IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: human Uterus Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC5.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Cardiac Muscle Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Lung Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC4.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Lung Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Lymph node Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC3.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Lymph node Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: human Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC2.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: human Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Sample: human Placenta Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21219_IHC.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human Placenta Tissue; Primary Ab: 20ug/ml Mouse Anti-human PIIINP Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-PIIINP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
138,564 Da
NCBI Official Full Name
collagen alpha-1(III) chain preproprotein
NCBI Official Synonym Full Names
collagen, type III, alpha 1
NCBI Official Symbol
COL3A1
NCBI Official Synonym Symbols
EDS4A
NCBI Protein Information
collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant
UniProt Protein Name
Collagen alpha-1(III) chain
UniProt Gene Name
COL3A1
UniProt Entry Name
CO3A1_HUMAN

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Product Notes

The PIIINP col3a1 (Catalog #AAA21219) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Gln24~Pro153. The Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP) reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Procollagen III N-Terminal Propeptide (PIIINP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the PIIINP col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Procollagen III N-Terminal Propeptide (PIIINP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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