WB (Western Blot)
(Detection of human Progesterone Receptor by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
Mouse anti-Human Progesterone Receptor Monoclonal Antibody | anti-PGR antibody
Mouse anti-Progesterone Receptor Monoclonal Antibody [188]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Progesterone Receptor by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Progesterone Receptor by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Progesterone Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1) used for IP at 6 ul per reaction. Progesterone Receptor was also immunoprecipitated by rabbit anti-Progesterone Receptor antibody A301-200A. For blotting immunoprecipitated Progesterone Receptor, AAA213506 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Progesterone Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1) used for IP at 6 ul per reaction. Progesterone Receptor was also immunoprecipitated by rabbit anti-Progesterone Receptor antibody A301-200A. For blotting immunoprecipitated Progesterone Receptor, AAA213506 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemisry)
(Detection of human Progesterone Receptor in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human Progesterone Receptor in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Progesterone Receptor in FFPE MCF-7 cells by immunocytochemistry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Progesterone Receptor in FFPE MCF-7 cells by immunocytochemistry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human Progesterone Receptor (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Progesterone Receptor (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Progesterone Receptor monoclonal antibody [188] (AAA213506) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)
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Product Notes
The PGR pgr (Catalog #AAA213506) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Progesterone Receptor Monoclonal Antibody [188] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Progesterone Receptor can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PGR pgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Progesterone Receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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