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product-image-AAA21233_WB6.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Spleen lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Heart lysate; Lane7: Caprine Liver lysate; Lane8: Gallus CerebuM lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Mouse Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody | anti-PCNA antibody

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)

Reactivity
Human, Mouse, Rat, Pig, Guinea pig, Goat, Sheep, Chicken
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Proliferating Cell Nuclear Antigen (PCNA), Antibody; Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA); Proliferating Cell Nuclear Antigen; Cyclin; anti-PCNA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat, Pig, Guinea pig, Goat, Sheep, Chicken
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C8
Specificity
The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Leu6~Ser261
Applicable Applications for anti-PCNA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-3ug/mL
IHC: 5-30ug/mL
ICC: 5-30ug/mL
Immunogen
Recombinant Proliferating Cell Nuclear Antigen (PCNA)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Spleen lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Heart lysate; Lane7: Caprine Liver lysate; Lane8: Gallus CerebuM lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_WB6.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Spleen lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Heart lysate; Lane7: Caprine Liver lysate; Lane8: Gallus CerebuM lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Lane1: Mouse Small intestine lysate; Lane2: Mouse Lymph node lysate; Lane3: Mouse Ovary lysate; Lane4: Mouse Thymus lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_WB5.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Mouse Small intestine lysate; Lane2: Mouse Lymph node lysate; Lane3: Mouse Ovary lysate; Lane4: Mouse Thymus lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Lane1: Sp2/0 cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_WB4.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Sp2/0 cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: YAC1 cell lysate; Lane7: MCF7 cell lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_WB3.jpg WB (Western Blot) (Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: YAC1 cell lysate; Lane7: MCF7 cell lysatePrimary Ab: 1ug/ml Mouse Anti-ulti-species PCNA AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot)

(Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Jurkat cell lysate; Lane3: Rat Testis lysate Primary Ab: 3ug/ml Mouse Anti-ulti-species PCNA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_WB2.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Jurkat cell lysate; Lane3: Rat Testis lysate Primary Ab: 3ug/ml Mouse Anti-ulti-species PCNA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Sample: human Breast cancer Tissue; Primary Ab: 20ug/ml Mouse Anti-ulti-species PCNA Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

product-image-AAA21233_IHC.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Sample: human Breast cancer Tissue; Primary Ab: 20ug/ml Mouse Anti-ulti-species PCNA Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-PCNA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,785 Da
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
Pcna
NCBI Protein Information
proliferating cell nuclear antigen; cyclin
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
Pcna
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_MOUSE

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Product Notes

The PCNA pcna (Catalog #AAA21233) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Leu6~Ser261. The Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) reacts with Human, Mouse, Rat, Pig, Guinea pig, Goat, Sheep, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Proliferating Cell Nuclear Antigen (PCNA) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-3ug/mL IHC: 5-30ug/mL ICC: 5-30ug/mL. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proliferating Cell Nuclear Antigen (PCNA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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