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product-image-AAA312616_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Daudi cells with Proteasome 20S LMP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

Rabbit anti-Human, Mouse Proteasome 20S LMP2 Monoclonal Antibody | anti-PSMB9 antibody

Proteasome 20S LMP2 Antibody

Gene Names
PSMB9; LMP2; PSMB6i; RING12; beta1i
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Proteasome 20S LMP2, Antibody; Proteasome 20S LMP2 Antibody; Beta1i antibody; Large multifunctional peptidase 2 antibody; Large multifunctional protease 2 antibody; LMP 2 antibody; LMP2 antibody; Low molecular mass protein 2 antibody; Macropain chain 7 antibody; MGC70470 antibody; Multicatalytic endopeptidase complex chain 7 antibody; OTTHUMP00000062982 antibody; Proteasome (prosome macropain) subunit beta type 9 antibody; proteasome (prosome; macropain) subunit; beta type; 9 (large multifunctional peptidase 2) antibody; Proteasome beta 9 subunit antibody; Proteasome catalytic subunit 1i antibody; Proteasome chain 7 antibody; Proteasome related gene 2 antibody; Proteasome subunit beta 6i antibody; Proteasome subunit beta type 9 antibody; Proteasome subunit beta type-9 antibody; Proteasome subunit beta-1i antibody; PSB9_HUMAN antibody; PSMB 9 antibody; PSMB6i antibody; PSMB9 antibody; Really interesting new gene 12 protein antibody; RING 12 antibody; RING12 antibody; RING12 protein antibody; anti-PSMB9 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JB35-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PSMB9 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Daudi cells with Proteasome 20S LMP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

product-image-AAA312616_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of Daudi cells with Proteasome 20S LMP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Proteasome 20S LMP2 antibody. Counter stained with hematoxylin.)

product-image-AAA312616_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Proteasome 20S LMP2 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse spleen tissue Lane 2: U937 Lane 2: Mouse colon tissue)

product-image-AAA312616_WB15.jpg WB (Western Blot) (Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse spleen tissue Lane 2: U937 Lane 2: Mouse colon tissue)
Related Product Information for anti-PSMB9 antibody
The eukaryotic multicatalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-gamma, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-gamma stimulation. LMP7 is also expressed as two forms, LMP7-E1 and E2, in several tissues.
Product Categories/Family for anti-PSMB9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
219
NCBI Official Full Name
Proteasome subunit beta type-9
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, beta type, 9
NCBI Official Symbol
PSMB9
NCBI Official Synonym Symbols
LMP2; PSMB6i; RING12; beta1i
NCBI Protein Information
proteasome subunit beta type-9; macropain chain 7; proteasome chain 7; proteasome-related gene 2; proteasome subunit beta 6i; proteasome subunit beta-1i; low molecular mass protein 2; proteasome catalytic subunit 1i; large multifunctional peptidase 2; rea
UniProt Protein Name
Proteasome subunit beta type-9
UniProt Gene Name
PSMB9
UniProt Synonym Gene Names
LMP2; PSMB6i; RING12
UniProt Entry Name
PSB9_HUMAN

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Product Notes

The PSMB9 psmb9 (Catalog #AAA312616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Proteasome 20S LMP2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Proteasome 20S LMP2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PSMB9 psmb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteasome 20S LMP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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