Mouse PROTEIN GENE PRODUCT 9.5 Monoclonal Antibody | anti-UCHL1 antibody

MOUSE ANTI HUMAN PROTEIN GENE PRODUCT 9.5

Cat. Num. AAA49206

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
• Reactivity: Rabbit, Rat; Does not react with: Guinea Pig; N.B. Antibody reactivity and working conditions may vary between species.
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: PROTEIN GENE PRODUCT 9.5, Antibody; MOUSE ANTI HUMAN PROTEIN GENE PRODUCT 9.5; anti-UCHL1 antibody

• Host: Mouse
• Reactivity: Rabbit, Rat; Does not react with: Guinea Pig; N.B. Antibody reactivity and working conditions may vary between species.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 31A3
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Approximate Protein Concentration: 1mg/ml (varies by lot)
• Sequence Length: 223

• Applicable Applications for anti-UCHL1 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Immunogen: Native, from brain
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Western blot analysis of A549 human alveolar adenocarcinoma whole cell lysate probed with Mouse anti Human PGP9.5 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)

Related Product Information for anti-UCHL1 antibody

Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway. Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein. Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs (Wilson et al. 1988). Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3 has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue (Gauntiz et al. 2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 24,824 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin thioesterase L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN