Application Data
(Western blot detection of PP2A-alpha(N-terminus) antibody in MCF7 cell lysates using PP2A-alpha(N-terminus) antibody (1:1000 diluted). Predicted band size:35KDa. Observed band size:35KDa.)
Mouse anti-Human protein phosphatase 2A Monoclonal Antibody | anti-PP2Calpha antibody
protein phosphatase 2A Antibody
Gene Names
PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Affinity purified
Synonyms
protein phosphatase 2A, Antibody; protein phosphatase 2A Antibody; RP-C; PP2Ac; PP2Calpha; Protein Phosphatase 2 alpha; anti-PP2Calpha antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Specificity
This antibody detects endogenous levels PP2A-alpha (N-terminus) and does not cross-react with related proteins.
Purity/Purification
Affinity purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Sequence Length
309
Applicable Applications for anti-PP2Calpha antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human PP2A-alpha (N-terminus) protein fragments expressed in E Coli.
Cellular Localization
Cytoplasm; Nucleus
Application Data
(Western blot detection of PP2A-alpha(N-terminus) antibody in MCF7 cell lysates using PP2A-alpha(N-terminus) antibody (1:1000 diluted). Predicted band size:35KDa. Observed band size:35KDa.)
Application Data
(Western blot detection of PP2A-alpha(N-terminus) antibody in MCF7 cell lysates using PP2A-alpha(N-terminus) antibody (1:1000 diluted). Predicted band size:35KDa. Observed band size:35KDa.)
Application Data
(Immunocytochemistry of HeLa cells using anti- PP2A-alpha (N-terminus) antibody diluted 1:50.)
Application Data
(Immunocytochemistry of HeLa cells using anti- PP2A-alpha (N-terminus) antibody diluted 1:50.)
Related Product Information for anti-PP2Calpha antibody
UniPort Summary Function: PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen
Entrez Summary: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Entrez Summary: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
Product Categories/Family for anti-PP2Calpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35kDa
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform 1
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit alpha
NCBI Official Symbol
PPP2CA
NCBI Official Synonym Symbols
RP-C; PP2Ac; PP2CA; PP2Calpha
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
UniProt Gene Name
PPP2CA
UniProt Synonym Gene Names
PP2A-alpha; RP-C
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Product Notes
The PP2Calpha ppp2ca (Catalog #AAA111275) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The protein phosphatase 2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's protein phosphatase 2A can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PP2Calpha ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "protein phosphatase 2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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