IF (Immunofluorescence)
(ICC/IF analysis of PSMA in LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human PSMA Monoclonal Antibody | anti-PSMA antibody
PSMA antibody
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
PSMA, Antibody; PSMA antibody; folate hydrolase (prostate-specific membrane antigen); FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA; glutamate carboxypeptidase 2 isoform 2; anti-PSMA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
k1H7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (determined by BCA assay) (varies by lot)
Sequence Length
719
Applicable Applications for anti-PSMA antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human PSMA (117-351aa) purified from E. coli
Preparation and Storage
Can be stored at +2°C to +8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PSMA in LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of PSMA in LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(Cell lysates of LNCaP (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMA (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(Cell lysates of LNCaP (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMA (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PSMA antibody
Prostate-specific membrane antigen (PSMA) is a 100-120 kDa type II transmembrane glycoprotein that is a glutamate-preferring carboxy-peptidase. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer.
Product Categories/Family for anti-PSMA antibody
References
Rajasekaran SA, et al., (2008) Mol Cancer Ther 7 (7):2142-2151; Polly D.G, et al., (2005) Int J Cancer 116 (3):415-421; Susan F.S, et al., (2005) Expert Opin Ther Targets 9 (3):561-570
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glutamate carboxypeptidase 2 isoform 2
NCBI Official Synonym Full Names
folate hydrolase 1
NCBI Official Symbol
FOLH1
NCBI Official Synonym Symbols
PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
NCBI Protein Information
glutamate carboxypeptidase 2
UniProt Protein Name
Glutamate carboxypeptidase 2
UniProt Gene Name
FOLH1
UniProt Synonym Gene Names
FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
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Product Notes
The PSMA folh1 (Catalog #AAA48014) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSMA antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PSMA folh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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