WB (Western Blot)
(PSMA5 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).)
Mouse PSMA5 Monoclonal Antibody | anti-PSMA5 antibody
PSMA5 Antibody
Gene Names
PSMA5; PSC5; ZETA
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Synonyms
PSMA5, Antibody; PSMA5 Antibody; Proteasome subunit alpha type-5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain; PSMA5; anti-PSMA5 antibody
Host
Mouse
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
426CT8.5.1
Specificity
Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
183
Applicable Applications for anti-PSMA5 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(PSMA5 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).)
WB (Western Blot)
(PSMA5 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. AAA283501 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. AAA283501 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody. AAA283501 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody. AAA283501 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-PSMA5 antibody
The proteasome is a multicatalytic proteinase complex with
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the peptidase T1A family, that is a 20S core alpha
subunit.
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the peptidase T1A family, that is a 20S core alpha
subunit.
Product Categories/Family for anti-PSMA5 antibody
References
Kottgen, A., et al. Nat. Genet. 42(5):376-384(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Hirano, Y., et al. Nature 437(7063):1381-1385(2005)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Hirano, Y., et al. Nature 437(7063):1381-1385(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26411
NCBI Official Full Name
proteasome subunit alpha type-5 isoform 2
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, alpha type, 5
NCBI Official Symbol
PSMA5
NCBI Official Synonym Symbols
PSC5; ZETA
NCBI Protein Information
proteasome subunit alpha type-5
UniProt Protein Name
Proteasome subunit alpha type-5
UniProt Gene Name
PSMA5
UniProt Entry Name
PSA5_HUMAN
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Product Notes
The PSMA5 psma5 (Catalog #AAA283501) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSMA5 Antibody reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PSMA5 psma5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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