WB (Western Blot)
(All lanes : Anti-RAB3B Antibody at 1:2000 dilutionLane 1: mouse brain lysateLane 2: rat brain lysateLane 3: SW480 whole cell lysateLane 4: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse RAB3B Monoclonal Antibody | anti-RAB3B antibody
RAB3B Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Synonyms
RAB3B, Antibody; RAB3B Antibody; Purified Mouse Monoclonal Antibody (Mab); Ras-related protein Rab-3B; RAB3B; anti-RAB3B antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
1543CT354.60.92
Specificity
This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B.
Purity/Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
219
Applicable Applications for anti-RAB3B antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:35871249). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:35871249).
Cellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus {ECO:0000250|UniProtKB:Q9CZT8}. Note=Colocalizes with GAS8/DRC4 in the Golgi apparatus. {ECO:0000250|UniProtKB:Q9CZT8}
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-RAB3B Antibody at 1:2000 dilutionLane 1: mouse brain lysateLane 2: rat brain lysateLane 3: SW480 whole cell lysateLane 4: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-RAB3B Antibody at 1:2000 dilutionLane 1: mouse brain lysateLane 2: rat brain lysateLane 3: SW480 whole cell lysateLane 4: human brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohiostchemistry)
(staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA290462 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290462, 1:25 dilution) for 60 min at 37 C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(at 1/400 dilution for 40 min at 37 C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA290462 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290462, 1:25 dilution) for 60 min at 37 C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(at 1/400 dilution for 40 min at 37 C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-RAB3B antibody
Protein transport. Probably involved in vesicular traffic (By similarity).
Product Categories/Family for anti-RAB3B antibody
References
Zahraoui A.,et al.J. Biol. Chem. 264:12394-12401(1989). Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. Gregory S.G.,et al.Nature 441:315-321(2006). Rigbolt K.T.,et al.Sci. Signal. 4:RS3-RS3(2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24758
NCBI Official Full Name
ras-related protein Rab-3B
NCBI Official Synonym Full Names
RAB3B, member RAS oncogene family
NCBI Official Symbol
RAB3B
NCBI Protein Information
ras-related protein Rab-3B
UniProt Protein Name
Ras-related protein Rab-3B
UniProt Gene Name
RAB3B
UniProt Entry Name
RAB3B_HUMAN
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Product Notes
The RAB3B rab3b (Catalog #AAA290462) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB3B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB3B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RAB3B rab3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB3B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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