FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with AAA48937 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human RAB7A Monoclonal Antibody | anti-RAB7A antibody
RAB7A antibody
Gene Names
RAB7A; RAB7; PRO2706
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
RAB7A, Antibody; RAB7A antibody; Ras-related protein Rab-7a; RAB7; anti-RAB7A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT10E4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (determined by BCA assay) (varies by lot)
Sequence Length
207
Applicable Applications for anti-RAB7A antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human RAB7A (1-207aa) purified from E. coli
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with AAA48937 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with AAA48937 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
IF (Immunofluorescence)
(ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with AAA48937 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with AAA48937 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysate Lane 5.: 293T cell lysate Lane 6.: SK-OV-3 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysate Lane 5.: 293T cell lysate Lane 6.: SK-OV-3 cell lysate)
Related Product Information for anti-RAB7A antibody
Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles.
References
Feng Y., et al. (1995) J Cell Biol. 131: 1435-52.; Barbero P., et al. (2002) J Cell Biol. 156: 511-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ras-related protein Rab-7a
NCBI Official Synonym Full Names
RAB7A, member RAS oncogene family
NCBI Official Symbol
RAB7A
NCBI Official Synonym Symbols
RAB7; PRO2706
NCBI Protein Information
ras-related protein Rab-7a
UniProt Protein Name
Ras-related protein Rab-7a
UniProt Gene Name
RAB7A
UniProt Synonym Gene Names
RAB7
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Product Notes
The RAB7A rab7a (Catalog #AAA48937) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB7A antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB7A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RAB7A rab7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB7A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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