Mouse RAC1 Monoclonal Antibody | anti-RAC1 antibody

RAC1 Antibody

Cat. Num. AAA283580

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1
• Reactivity: Human, rat (Predicted Reactivity: Bovine, Mouse)
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: RAC1, Antibody; RAC1 Antibody; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1; RAC1; TC25; anti-RAC1 antibody

• Host: Mouse
• Reactivity: Human, rat (Predicted Reactivity: Bovine, Mouse)
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1301CT276.121.104
• Specificity: This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 192

• Applicable Applications for anti-RAC1 antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles (PubMed:1643658, PubMed:28886345). Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity (PubMed:9121475). In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts (PubMed:1643658). In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity (By similarity). In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX (PubMed:12695502). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization (By similarity).
• Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P63001}. Cell projection, dendrite {ECO:0000250|UniProtKB:P63001}. Synapse {ECO:0000250|UniProtKB:Q6RUV5} Nucleus. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity). In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity) Activation by GTP-binding promotes nuclear localization (PubMed:12551911). {ECO:0000250|UniProtKB:P63001, ECO:0000250|UniProtKB:Q6RUV5, ECO:0000269|PubMed:12551911, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:1903399.
• Tissue Location: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysates from A431, mouse NIH/3T3, rat C6 cell line (from left to right), using RAC1 Antibody. AAA283580 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded H.stomach section using RAC1. AAA283580 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded H.skin section using RAC1. AAA283580 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of U-87 MG cells using RAC1(green) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

Product Categories/Family for anti-RAC1 antibody

Crown Antibodies; Cancer; Cell Biology; Metabolism; Amyotrophic Lateral Sclerosis (ALS)

References

Didsbury J.,et al.J. Biol. Chem. 264:16378-16382(1989).
Drivas G.T.,et al.Mol. Cell. Biol. 10:1793-1798(1990).
Matos P.,et al.Biochem. Biophys. Res. Commun. 277:741-751(2000).
Jordan P.,et al.Oncogene 18:6835-6839(1999).
Schnelzer A.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 9845511
• NCBI GeneID: 5879
• NCBI Accession #: NP_008839.2
• NCBI GenBank Nucleotide #: NM_006908.4
• UniProt Accession #: P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4; AAB22206
• Molecular Weight: 21450
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 1 isoform Rac1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25
• UniProt Entry Name: RAC1_HUMAN