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FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit RAD51 Monoclonal Antibody | anti-RAD51 antibody
RAD51 Antibody
Gene Names
RAD51; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
RAD51; Monoclonal Antibody; RAD51 Antibody; BRCA1/BRCA2 containing complex; subunit 5 antibody; BRCC 5 antibody; BRCC5 antibody; DNA repair protein RAD51 homolog 1 antibody; DNA repair protein rhp51 antibody; FANCR antibody; hRAD51 antibody; HsRAD51 antibody; HsT16930 antibody; MRMV2 antibody; Rad 51 antibody; RAD51 antibody; RAD51 homolog (RecA homolog; E. coli) (S. cerevisiae) antibody; RAD51 homolog A antibody; RAD51 homolog antibody; RAD51 recombinase antibody; S. cerevisiae; homolog of antibody; RAD51_HUMAN antibody; RAD51A antibody; RECA antibody; RecA like protein antibody; RecA; E. coli; Recombination protein A antibody; anti-RAD51 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JM54-26
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RAD51 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with Rad51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining Rad51 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Rad51 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Rad51 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Rad51 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Rad51 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Rad51 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Rad51 on mouse testie tissue lytase using anti-Rad51 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of Rad51 on mouse testie tissue lytase using anti-Rad51 antibody at 1/1, 000 dilution.)
Related Product Information for anti-RAD51 antibody
Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. MRE11 (meiotic recombination 11, ATLD, HNGS1) is a nuclear 3-5 exonuclease/endonuclease that associates with Rad50 and influences homologous recombination, telomere length maintenance, and DNA double-strand break repair. MRE11 is most abundant in proliferating tissues.
Product Categories/Family for anti-RAD51 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,966 Da
NCBI Official Full Name
DNA repair protein RAD51 homolog 1 isoform 2
NCBI Official Synonym Full Names
RAD51 homolog (S. cerevisiae)
NCBI Official Symbol
RAD51
NCBI Official Synonym Symbols
RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
NCBI Protein Information
DNA repair protein RAD51 homolog 1; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; BRCA1/BRCA2-containing complex, subunit 5
UniProt Protein Name
DNA repair protein RAD51 homolog 1
UniProt Gene Name
RAD51
UniProt Synonym Gene Names
RAD51A; RECA; HsRAD51; hRAD51
UniProt Entry Name
RAD51_HUMAN
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Product Notes
The RAD51 rad51 (Catalog #AAA30389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAD51 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAD51 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RAD51 rad51 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD51, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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