Mouse RAD51 Monoclonal Antibody | anti-RAD51 antibody

RAD51 (Prognostic and Response to Chemotherapy Marker)

Cat. Num. AAA214806

• Gene Names: RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: RAD51, Antibody; RAD51 (Prognostic and Response to Chemotherapy Marker); RCA1/BRCA2 containing complex, subunit 5; BRCC5; DNA repair protein RAD51; FANCR; HsT16930; MRMV2; RAD51; RAD51 recombinase; RAD51A; RECA; RecA like protein; Recombination protein A; anti-RAD51 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: RAD51/2702
• Specificity: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies. RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 242

• Applicable Applications for anti-RAD51 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)
• Positive Control: HeLa or MCF-7 Cells. Colon or Breast Carcinoma
• Cellular Localization: Nuclear
• Chromosome Location: 15q15.1
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using RAD51 Mouse Monoclonal Antibody (RAD51/2702). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RAD51 Mouse Monoclonal Antibody (RAD51/2702).)

References

Henning W, Stuerzbecher H-W (2003) Homologous recombination and cell cycle checkpoints: Rad51 in tumour progression and therapy resistance. Toxicology 193: 91-109

NCBI and Uniprot Product Information

• NCBI GI #: 256017143
• NCBI GeneID: 5888
• NCBI Accession #: NP_001157741.1
• NCBI GenBank Nucleotide #: NM_001164269.1
• UniProt Accession #: Q06609; Q6FHX9; Q6ZNA8; Q9BV60; B0FXP0; B2R8T6
• Molecular Weight: 37kDa
• NCBI Official Full Name: DNA repair protein RAD51 homolog 1 isoform 2
• NCBI Official Synonym Full Names: RAD51 recombinase
• NCBI Official Symbol: RAD51
• NCBI Official Synonym Symbols: RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• NCBI Protein Information: DNA repair protein RAD51 homolog 1
• UniProt Protein Name: DNA repair protein RAD51 homolog 1
• UniProt Gene Name: RAD51
• UniProt Synonym Gene Names: RAD51A; RECA; HsRAD51; hRAD51