IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RAD51B protein using RAD51B antibody)
Mouse RAD51B Monoclonal Antibody | anti-RAD51B antibody
RAD51B antibody
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
RAD51B antibody was purified by affinity chromatography.
Synonyms
RAD51B, Antibody; RAD51B antibody; Monoclonal RAD51B; Anti-RAD51B; RAD51 like 1; R51H2; REC2; hREC2; MGC34245; DNA repair protein RAD51 homolog 2; Recombination repair protein; RAD51B; RADB 51; RADB-51; anti-RAD51B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H4
Purity/Purification
RAD51B antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Applicable Applications for anti-RAD51B antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
RAD51B is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, whichsuggested a role of this protein in sensing DNA damage.
Cross-Reactivity
Human
Immunogen
RAD51B antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1 (NP_598193) produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RAD51B protein using RAD51B antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant RAD51B protein using RAD51B antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RAD51B protein (Right) or empty vector (Left) detected with RAD51B antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RAD51B protein (Right) or empty vector (Left) detected with RAD51B antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RAD51B protein (red) or empty vector (blue) stained using RAD51B antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RAD51B protein (red) or empty vector (blue) stained using RAD51B antibody)
Related Product Information for anti-RAD51B antibody
Mouse monoclonal RAD51B antibody
Product Categories/Family for anti-RAD51B antibody
Similar Products
Product Notes
The RAD51B (Catalog #AAA108153) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAD51B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the RAD51B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD51B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.