FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human RalA/RalB Monoclonal Antibody | anti-RalA antibody
RalA/RalB
Gene Names
RALA; RAL
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
RalA/RalB, Antibody; RalA/RalB; Ras-related protein Ral-A; RAL; Ras-related protein Ral-B; anti-RalA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT41E2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
206
Applicable Applications for anti-RalA antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human RALA (1-203aa) purified from E. coli
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human RalA protein Lane 2.: Recombinant human RalB protein)
WB (Western Blot)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human RalA protein Lane 2.: Recombinant human RalB protein)
Related Product Information for anti-RalA antibody
RalA and RalB belong to subfamily of Ras-related GTPases. This protein mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. It is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicletrafficking, and endocytosis. RalA and RalB share 85% identity.
Product Categories/Family for anti-RalA antibody
References
Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79.; Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.; Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; Jullien-Flores V., et al. (1995). J. Biol. Chem. 270: 22473-22477.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-related protein Ral-A
NCBI Official Synonym Full Names
RAS like proto-oncogene A
NCBI Official Symbol
RALA
NCBI Official Synonym Symbols
RAL
NCBI Protein Information
ras-related protein Ral-A
UniProt Protein Name
Ras-related protein Ral-A
UniProt Gene Name
RALA
UniProt Synonym Gene Names
RAL
UniProt Entry Name
RALA_HUMAN
Similar Products
Product Notes
The RalA rala (Catalog #AAA48353) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RalA/RalB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RalA/RalB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the RalA rala for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RalA/RalB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.