IP (Immunoprecipitation)
(TNFSF11 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate0.5 uL anti-TNFSF11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TNFSF11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 40 kDa)
Rabbit anti-Human RANKL Monoclonal Antibody | anti-TNFSF11 antibody
Recombinant Anti-RANKL Antibody, Rabbit Monoclonal
Gene Names
TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
Reactivity
Human
Applications
Immunoprecipitation, ELISA, ELISA, Western Blot
Purity
Protein A
Synonyms
RANKL, Antibody; Recombinant Anti-RANKL Antibody, Rabbit Monoclonal; RANKL/OPGL/TNFSF11/CD254 Antibody, Rabbit MAb; tumor necrosis factor (ligand) superfamily, member 11; Anti-CD254 Antibody; Anti-hRANKL2 Antibody; Anti-ODF Antibody; Anti-OPGL Antibody; Anti-OPTB2 Antibody; Anti-RANKL Antibody; Anti-sOdf Antibody; Anti-TRANCE Antibody; anti-TNFSF11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
101
Specificity
Human RANKL/OPGL/TNFSF11/CD254
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNFSF11 antibody
IP (Immunoprecipitation), ELISA, ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human RANKL/OPGL/TNFSF11/CD254 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human RANKL/OPGL/TNFSF11/CD254 (rh RANKL/OPGL/TNFSF11/CD254).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(TNFSF11 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate0.5 uL anti-TNFSF11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TNFSF11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 40 kDa)
IP (Immunoprecipitation)
(TNFSF11 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate0.5 uL anti-TNFSF11 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TNFSF11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 40 kDa)
WB (Western Blot)
(Anti-TNFSF11 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa)
WB (Western Blot)
(Anti-TNFSF11 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa)
Related Product Information for anti-TNFSF11 antibody
Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein that belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.
Product Categories/Family for anti-TNFSF11 antibody
References
Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.
Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.
Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
47.1 kDa
NCBI Official Full Name
TRANCE, partial
NCBI Official Synonym Full Names
TNF superfamily member 11
NCBI Official Symbol
TNFSF11
NCBI Official Synonym Symbols
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
NCBI Protein Information
tumor necrosis factor ligand superfamily member 11
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 11
UniProt Gene Name
TNFSF11
UniProt Synonym Gene Names
OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE
UniProt Entry Name
TNF11_HUMAN
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Product Notes
The TNFSF11 tnfsf11 (Catalog #AAA255461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-RANKL Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RANKL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TNFSF11 tnfsf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RANKL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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