FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse anti-Human RAP1GAP Monoclonal Antibody | anti-RAP1GAP antibody
RAP1GAP Antibody
Gene Names
RAP1GAP; RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
ProA affinity purified
Synonyms
RAP1GAP, Antibody; RAP1GAP Antibody; KIAA0474 antibody; Rap1 GTPase activating protein 1 antibody; RAP1 GTPase activating protein antibody; Rap1 GTPase-activating protein 1 antibody; RAP1GA1 antibody; Rap1ga1 protein antibody; Rap1GAP antibody; Rap1GAP1 antibody; RAP1GAPII antibody; RAPGAP antibody; RPGP1_HUMAN antibody; anti-RAP1GAP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
B6-G7
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RAP1GAP antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
WB (Western Blot)
(Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1, 000 dilution.)
Related Product Information for anti-RAP1GAP antibody
Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Galpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated Galpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Galpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
Product Categories/Family for anti-RAP1GAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,361 Da
NCBI Official Full Name
rap1 GTPase-activating protein 1 isoform b
NCBI Official Synonym Full Names
RAP1 GTPase activating protein
NCBI Official Symbol
RAP1GAP
NCBI Official Synonym Symbols
RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
NCBI Protein Information
rap1 GTPase-activating protein 1
UniProt Protein Name
Rap1 GTPase-activating protein 1
UniProt Gene Name
RAP1GAP
UniProt Synonym Gene Names
KIAA0474; RAP1GA1; Rap1GAP; Rap1GAP1
UniProt Entry Name
RPGP1_HUMAN
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Product Notes
The RAP1GAP rap1gap (Catalog #AAA312032) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAP1GAP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP1GAP can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the RAP1GAP rap1gap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP1GAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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