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ICC (Immunocytochemistry)
(ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit RAS Monoclonal Antibody | anti-RAS antibody
RAS Antibody
Gene Names
NRAS; NS6; ALPS4; N-ras; NRAS1
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
RAS, Antibody; RAS Antibody; C-BAS/HAS antibody; c-H-ras antibody; C-HA-RAS1 antibody; CTLO antibody; GTPase HRas antibody; GTPase KRas antibody; GTPase NRas antibody; H-Ras-1 antibody; H-RASIDX antibody; Ha-Ras antibody; HAMSV antibody; HRAS antibody; HRAS1 antibody; K RAS2A antibody; K RAS2B antibody; K RAS4A antibody; K RAS4B antibody; K-RAS antibody; KRAS antibody; KRAS1 antibody; KRAS2 antibody; N-RAS antibody; N-terminally processed antibody; NRAS antibody; NRAS1 antibody; p21ras antibody; RASH_HUMAN antibody; RASH1 antibody; RASK2 antibody; Transforming protein p21 antibody; v Ha ras Harvey rat sarcoma viral oncogene homolog antibody; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody; v ras neuroblastoma RAS viral oncogene homolog antibody; anti-RAS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
JF10-11
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RAS antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Ras in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Ras in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: MCF-7 Lane 3: Hela Lane 4: zebrafish)
WB (Western Blot)
(Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: MCF-7 Lane 3: Hela Lane 4: zebrafish)
Related Product Information for anti-RAS antibody
The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.
Product Categories/Family for anti-RAS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21kDa
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
neuroblastoma RAS viral (v-ras) oncogene homolog
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; ALPS4; N-ras; NRAS1
NCBI Protein Information
GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog
UniProt Protein Name
GTPase NRas
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1
UniProt Entry Name
RASN_HUMAN
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Product Notes
The RAS nras (Catalog #AAA312443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAS Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's RAS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RAS nras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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