FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Raji cells using Anti-RASSF2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
Mouse anti-Human RASSF2 Monoclonal Antibody | anti-RASSF2 antibody
Anti-RASSF2 Antibody
Gene Names
RASSF2; CENP-34; RASFADIN
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
This antibody is purified through a protein G column.
Synonyms
RASSF2, Antibody; Anti-RASSF2 Antibody; anti-RASSF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Specificity
Recognizes endogenous levels of RASSF2 protein.
Purity/Purification
This antibody is purified through a protein G column.
Form/Format
Mouse IgG2a kappa. Liquid in PBS, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-RASSF2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Recombinant fusion protein of human RASSF2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Raji cells using Anti-RASSF2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Raji cells using Anti-RASSF2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
WB (Western Blot)
(Western blot analysis of RASSF2 expression in Raji (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 38 kD))
WB (Western Blot)
(Western blot analysis of RASSF2 expression in Raji (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 38 kD))
Related Product Information for anti-RASSF2 antibody
Mouse monoclonal antibody to RASSF2
Product Categories/Family for anti-RASSF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,879 Da
NCBI Official Full Name
ras association domain-containing protein 2
NCBI Official Synonym Full Names
Ras association (RalGDS/AF-6) domain family member 2
NCBI Official Symbol
RASSF2
NCBI Official Synonym Symbols
CENP-34; RASFADIN
NCBI Protein Information
ras association domain-containing protein 2; centromere protein 34; Ras association (RalGDS/AF-6) domain family 2
UniProt Protein Name
Ras association domain-containing protein 2
UniProt Gene Name
RASSF2
UniProt Synonym Gene Names
KIAA0168
UniProt Entry Name
RASF2_HUMAN
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Product Notes
The RASSF2 rassf2 (Catalog #AAA266038) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RASSF2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RASSF2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the RASSF2 rassf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASSF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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