ICC (Immunocytochemistry)
(Immunocytochemistry of Rb(green) in Hela cells using Rb Rabbit mAb at dilution 1/200, and DAPI(blue))
anti-Human Rb Monoclonal Antibody | anti-Rb antibody
Rb Rabbit mAb
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
Rb, Antibody; Rb Rabbit mAb; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; anti-Rb antibody
Host
Recombinant Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
S09-4H8
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-Rb antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthetic peptide of human Rb
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
ICC (Immunocytochemistry)
(Immunocytochemistry of Rb(green) in Hela cells using Rb Rabbit mAb at dilution 1/200, and DAPI(blue))
ICC (Immunocytochemistry)
(Immunocytochemistry of Rb(green) in Hela cells using Rb Rabbit mAb at dilution 1/200, and DAPI(blue))
WB (Western Blot)
(Western blot detection of Rb in 3T3 cell lysates using Rb Rabbit mAb(1:1000 diluted).Predicted band size:106kDa.Observed band size:110kDa.)
WB (Western Blot)
(Western blot detection of Rb in 3T3 cell lysates using Rb Rabbit mAb(1:1000 diluted).Predicted band size:106kDa.Observed band size:110kDa.)
Related Product Information for anti-Rb antibody
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex .
Product Categories/Family for anti-Rb antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106,159 Da
NCBI Official Full Name
retinoblastoma-associated protein
NCBI Official Synonym Full Names
retinoblastoma 1
NCBI Official Symbol
RB1
NCBI Official Synonym Symbols
RB; pRb; OSRC; pp110; p105-Rb
NCBI Protein Information
retinoblastoma-associated protein; retinoblastoma suspectibility protein
UniProt Protein Name
Retinoblastoma-associated protein
UniProt Gene Name
RB1
UniProt Synonym Gene Names
Rb
UniProt Entry Name
RB_HUMAN
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Product Notes
The Rb rb1 (Catalog #AAA314450) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rb Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rb can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Rb rb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rb, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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