IF (Immunofluorescence)
(Immunofluorescent analysis of RBBP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Mouse RBBP5 Monoclonal Antibody | anti-RBBP5 antibody
Anti-RBBP5 Antibody
Gene Names
RBBP5; RBQ3; SWD1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RBBP5, Antibody; Anti-RBBP5 Antibody; anti-RBBP5 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Specificity
Recognizes endogenous levels of RBBP5 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH7.3.
Applicable Applications for anti-RBBP5 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human RBBP5 protein fragments expressed in E coli.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of RBBP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of RBBP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of RBBP5 expression in C2C12 (A), THP1 (B), PC12 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD))
WB (Western Blot)
(Western blot analysis of RBBP5 expression in C2C12 (A), THP1 (B), PC12 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD))
Related Product Information for anti-RBBP5 antibody
Mouse monoclonal antibody to RBBP5
Product Categories/Family for anti-RBBP5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,153 Da
NCBI Official Full Name
retinoblastoma-binding protein 5 isoform 2
NCBI Official Synonym Full Names
retinoblastoma binding protein 5
NCBI Official Symbol
RBBP5
NCBI Official Synonym Symbols
RBQ3; SWD1
NCBI Protein Information
retinoblastoma-binding protein 5; RBBP-5; retinoblastoma-binding protein RBQ-3; SWD1, Set1c WD40 repeat protein, homolog
UniProt Protein Name
Retinoblastoma-binding protein 5
UniProt Gene Name
RBBP5
UniProt Synonym Gene Names
RBQ3; RBBP-5
UniProt Entry Name
RBBP5_HUMAN
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Product Notes
The RBBP5 rbbp5 (Catalog #AAA265799) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RBBP5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RBBP5 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the RBBP5 rbbp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBBP5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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