IF (Immunofluorescence)
(Immunofluorescence analysis of RelB was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton„¢ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti RelB antibody at 1.0 ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with an Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody for 45 minutes at room temperature (Panel A: green). Nuclei (Panel B: blue) were counterstained with DAPI and F-actin (Panel C: red) with rhodamine phalloidin. Panel D is a merged image showing nuclear localization. Panel E is a no primary antibody control. The images were captured at 60x magnification)
Mouse RelB Monoclonal Antibody | anti-RELB antibody
Mouse anti RelB
Gene Names
RELB; IREL; I-REL; IMD53; REL-B
Reactivity
Human, Rhesus Monkey, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry
Purity
Purified by affinity chromatography on Protein A
Synonyms
RelB, Antibody; Mouse anti RelB; RelB Antibody; anti-RELB antibody
Host
Mouse
Reactivity
Human, Rhesus Monkey, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
17.3
Purity/Purification
Purified by affinity chromatography on Protein A
Form/Format
Liquid in phosphate buffered saline with 0.1% sodium azide (NaN3).
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
579
Applicable Applications for anti-RELB antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
Recombinant protein derived from C-terminal region of human RelB protein
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
IF (Immunofluorescence)
(Immunofluorescence analysis of RelB was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton„¢ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti RelB antibody at 1.0 ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with an Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody for 45 minutes at room temperature (Panel A: green). Nuclei (Panel B: blue) were counterstained with DAPI and F-actin (Panel C: red) with rhodamine phalloidin. Panel D is a merged image showing nuclear localization. Panel E is a no primary antibody control. The images were captured at 60x magnification)
IF (Immunofluorescence)
(Immunofluorescence analysis of RelB was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton„¢ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Mouse anti RelB antibody at 1.0 ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with an Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody for 45 minutes at room temperature (Panel A: green). Nuclei (Panel B: blue) were counterstained with DAPI and F-actin (Panel C: red) with rhodamine phalloidin. Panel D is a merged image showing nuclear localization. Panel E is a no primary antibody control. The images were captured at 60x magnification)
WB (Western Blot)
(Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB at 1-2 ug/ml in 5 % skimmed milk at 4 degree C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection)
WB (Western Blot)
(Western blot analysis of RelB was performed by loading 20 ug of THP-1 (lane1), Daudi (lane2) and Raji (lane3) cell lysate on a gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skimmed milk for 1 hour at room temperature. RelB was detected at ~ 63 kDa using Mouse anti RelB at 1-2 ug/ml in 5 % skimmed milk at 4 degree C overnight on a rocking platform. A goat anti-mouse HRP conjugated secondary antibody was used for detection)
Related Product Information for anti-RELB antibody
Mouse anti-RelB antibody, clone 17.3 recognizes the transcription factor RelB, also known as I-Rel. RelB is encoded by the v-rel avian reticuloendotheliosis viral oncogene homolog B gene and is a critical component of the NF-kappa-B pathway.
Upon cleavage of the NF-kappa-B p105 and p100 subunits into the NF-kappa-B p50 and p52 subunits, RelB forms RelB-p50 and RelB-p52 heterodimers. These dimers act as activators of transcription by binding to kappaB sites present on DNA (Oeckinghaus and Ghosh 2009). RelB-p50 and RelB-p52 binding induces the expression of genes mediating immune responses, inflammation and apoptosis (Jing and Lee 2014).
Misregulation of the NF-kappa-B pathway has been implicated in a variety of disease states including autoimmunity and cancer. As the NF-kappa-B pathway has been demonstrated to be constitutively active in a large group of tumors, developing NF-kappa-B inhibitors has emerged as a new cancer therapy approach (Chaturverdi et al. 2011 and Baud and Karin et al. 2009).
Upon cleavage of the NF-kappa-B p105 and p100 subunits into the NF-kappa-B p50 and p52 subunits, RelB forms RelB-p50 and RelB-p52 heterodimers. These dimers act as activators of transcription by binding to kappaB sites present on DNA (Oeckinghaus and Ghosh 2009). RelB-p50 and RelB-p52 binding induces the expression of genes mediating immune responses, inflammation and apoptosis (Jing and Lee 2014).
Misregulation of the NF-kappa-B pathway has been implicated in a variety of disease states including autoimmunity and cancer. As the NF-kappa-B pathway has been demonstrated to be constitutively active in a large group of tumors, developing NF-kappa-B inhibitors has emerged as a new cancer therapy approach (Chaturverdi et al. 2011 and Baud and Karin et al. 2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,134 Da
NCBI Official Full Name
Transcription factor RelB
NCBI Official Synonym Full Names
RELB proto-oncogene, NF-kB subunit
NCBI Official Symbol
RELB
NCBI Official Synonym Symbols
IREL; I-REL; IMD53; REL-B
NCBI Protein Information
transcription factor RelB
UniProt Protein Name
Transcription factor RelB
UniProt Gene Name
RELB
Similar Products
Product Notes
The RELB relb (Catalog #AAA50314) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti RelB reacts with Human, Rhesus Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RelB can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the RELB relb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RelB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.