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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human Renin Monoclonal Antibody | anti-REN antibody
Recombinant Anti-Renin Antibody, Rabbit Monoclonal
Gene Names
REN; HNFJ2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A
Synonyms
Renin, Antibody; Recombinant Anti-Renin Antibody, Rabbit Monoclonal; Renin/REN/Angiotensinogenase Antibody, Rabbit MAb; renin; Anti-FLJ10761 Antibody; Anti-HNFJ2 Antibody; Anti-REN Antibody; anti-REN antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
009
Specificity
Human Renin/REN/Angiotensinogenase.
Has cross-reactivity in ELISA with Mouse REN1/Renin-1
Has cross-reactivity in ELISA with Mouse REN1/Renin-1
Has cross-reactivity in ELISA with Mouse REN1/Renin-1
Has cross-reactivity in ELISA with Mouse REN1/Renin-1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-REN antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human Renin protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (rh Renin).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
WB (Western Blot)
(Anti-RENIN rabbit monoclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:57 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-RENIN rabbit monoclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:57 kDa(We are unsure as to the identity of these extra bands.))
IHC (Immunohiostchemistry)
(Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (1:300) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (1:300) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Related Product Information for anti-REN antibody
Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.
References
McKeon F.D., et al., (1986), Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. Nature 319:463-468.Fisher D.Z., et al., (1986), cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.Proc. Natl. Acad. Sci. U.S.A. 83:6450-6454.Sylvius N., et al., (2005), In vivo and in vitro examination of the functional significances of novel lamin gene mutations in heart failure patients.J. Med. Genet. 42:639-647.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,057 Da
NCBI Official Full Name
renin preproprotein
NCBI Official Synonym Full Names
renin
NCBI Official Symbol
REN
NCBI Official Synonym Symbols
HNFJ2
NCBI Protein Information
renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
UniProt Protein Name
Renin
UniProt Gene Name
REN
UniProt Entry Name
RENI_HUMAN
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Product Notes
The REN ren (Catalog #AAA255266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Renin Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Renin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the REN ren for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Renin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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