<
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Mouse anti-Human RF1 Monoclonal Antibody | anti-RF1 antibody
RF1 Antibody
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
ProA affinity purified
Synonyms
RF1, Antibody; RF1 Antibody; Cl1 protein antibody; D5S1995 antibody; ERF antibody; eRF1 antibody; ERF1_HUMAN antibody; ETF1 antibody; Eukaryotic peptide chain release factor subunit 1 antibody; Eukaryotic release factor 1 antibody; Eukaryotic translation termination factor 1 antibody; MGC111066 antibody; Polypeptide chain release factor 1 antibody; Protein Cl1 antibody; RF1 antibody; Sup45 (yeast omnipotent suppressor 45) homolog like 1 antibody; SUP45L1 antibody; TB3 1 antibody; TB3-1 antibody; anti-RF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RF1 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
WB (Western Blot)
(Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: T47D Lane3: MOLT4 Lane4: Raji)
WB (Western Blot)
(Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: T47D Lane3: MOLT4 Lane4: Raji)
WB (Western Blot)
(Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-RF1 antibody
Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the "GTP state, " it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the "GDP state, " it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.
Product Categories/Family for anti-RF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,463 Da
NCBI Official Full Name
eukaryotic peptide chain release factor subunit 1 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation termination factor 1
NCBI Official Symbol
ETF1
NCBI Official Synonym Symbols
ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
NCBI Protein Information
eukaryotic peptide chain release factor subunit 1; polypeptide chain release factor 1; protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1
UniProt Protein Name
Eukaryotic peptide chain release factor subunit 1
UniProt Gene Name
ETF1
UniProt Synonym Gene Names
ERF1; RF1; SUP45L1; Eukaryotic release factor 1; eRF1
UniProt Entry Name
ERF1_HUMAN
Similar Products
Product Notes
The RF1 etf1 (Catalog #AAA312055) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RF1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the RF1 etf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.