Mouse RFP Tag Monoclonal Antibody | anti-RFP antibody

RFP Tag Mouse Monoclonal Antibody (RF5R)

Cat. Num. AAA14614

• Applications: Immunohistochemistry, Western Blot
• Synonyms: RFP Tag, Antibody; RFP Tag Mouse Monoclonal Antibody (RF5R); anti-RFP antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RF5R
• Specificity: The antibody recognizes native and denatured forms of RFP and its variants: TagRFR TurboRFR dsRed, mCherry, mOrange, tdTomato, etc
• Form/Format: Lyophilized protein G purified in PBS pH7.4

• Applicable Applications for anti-RFP antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: If reconstituted with deionized water in 100 uL: WB: 1:1,000-3,000; IHC: 1:500-2,000.; Optimal dilution has to be determined by the user.
• Immunogen: The antibody was raised against RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated.
• Target: RFP Tag
• Preparation and Storage: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

WB (Western Blot)

(Western blot analysis of HEK293 cells transfected with RFP-tagged protein vector. 1:1000 (1ug/mL) Ab dilution. Non·-transfected (1). transfected with TurboRFP (2) and transfected with dsRed (3))

Related Product Information for anti-RFP antibody

Target background: Red Fluorescent Protein (RFP) is a protein derived from the Discosoma sea anemone. Fluorescent proteins are powerful tools to study protein localization and dynamics in living cells. The anti-RFP antibody can be used to detect native RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange and tdTomato.