SDS-PAGE
(The purity of RFP was determined to be >90% by densitometry. Approx. MW 29 kDa)
RFP recombinant protein
RFP Protein
Purity
>90%
Synonyms
RFP; N/A; RFP Protein; drFP583; DsRed; RFP recombinant protein
Host
E Coli
Purity/Purification
>90%
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Tag
HIS
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Stability: 1 year at -70 degrees from date of receipt.
Stability: 1 year at -70 degrees from date of receipt.
SDS-PAGE
(The purity of RFP was determined to be >90% by densitometry. Approx. MW 29 kDa)
SDS-PAGE
(The purity of RFP was determined to be >90% by densitometry. Approx. MW 29 kDa)
Related Product Information for RFP recombinant protein
Recombinant full-length Sea anemone RFP was expressed in E Coli cells using an N-terminal His tag. The RFP protein accession number is AAF03369.
RFP (red fluorescent protein drFP583) has the protection role from the UV light and the conversion of suitable light for the microalgal symbionts in the coral reefs (1). The RFP proteins can be used in multicolor tagging experiments, avoiding natural cellular autofluorescence, and the resonance energy transfer-based experiments (2). The broad fluorescence emission spectrum peaks is at 583 nm (3).
RFP (red fluorescent protein drFP583) has the protection role from the UV light and the conversion of suitable light for the microalgal symbionts in the coral reefs (1). The RFP proteins can be used in multicolor tagging experiments, avoiding natural cellular autofluorescence, and the resonance energy transfer-based experiments (2). The broad fluorescence emission spectrum peaks is at 583 nm (3).
Product Categories/Family for RFP recombinant protein
References
1. Matz, M.V., et al.: Fluorescent proteins from non-bioluminescent Anthozoa species. Nat. Biotechnol. 17 (10), 969-973, 1999.
2. Yarbrough D., et al.: Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution. Proc Natl Acad Sci USA.; 98(2):462-7, 2001.
3. http://www.uniprot.org/uniprot/Q9U6Y8.
2. Yarbrough D., et al.: Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution. Proc Natl Acad Sci USA.; 98(2):462-7, 2001.
3. http://www.uniprot.org/uniprot/Q9U6Y8.
NCBI and Uniprot Product Information
Molecular Weight
~29kDa
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Product Notes
The RFP (Catalog #AAA73946) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RFP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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