WB (Western Blot)
(Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.)
Mouse Rhodopsin / RHO Monoclonal Antibody | anti-RHO antibody
Anti-Rhodopsin / RHO Antibody (clone B630) IHC-plus
Gene Names
RHO; RP4; OPN2; CSNBAD1
Reactivity
Mouse, Bovine, Rat, Pig, Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein G purified
Synonyms
Rhodopsin / RHO, Antibody; Anti-Rhodopsin / RHO Antibody (clone B630) IHC-plus; Mouse Monoclonal [clone B630] (IgG1,k) to Human Rhodopsin / RHO; Human Rhodopsin / RHO; CSNBAD1; OPN2; Rhodopsin; RP4; Opsin 2; rod pigment; Opsin-2; anti-RHO antibody
Host
Mouse
Reactivity
Mouse, Bovine, Rat, Pig, Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
B630
Purity/Purification
Protein G purified
Form/Format
PBS, 10 mM sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
348
Applicable Applications for anti-RHO antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Target Species
Human
Preparation and Storage
4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.)
WB (Western Blot)
(Blot of bovine retinal extracts probed with Rhodopsin / RHO antibody. The antibody stains a band corresponding to retinal rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.)
ICC (Immunocytochemistry)
(High magnification confocal image of pig retinal section stained with Rhodopsin / RHO antibody (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).)
ICC (Immunocytochemistry)
(High magnification confocal image of pig retinal section stained with Rhodopsin / RHO antibody (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).)
IHC (Immunohistochemistry)
(Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Related Product Information for anti-RHO antibody
RHO Antibody, CSNBAD1 Antibody, OPN2 Antibody, Rhodopsin Antibody, RP4 Antibody, Opsin 2, rod pigment Antibody, Opsin-2 Antibody Description: Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,893 Da
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin
NCBI Official Symbol
RHO
NCBI Official Synonym Symbols
RP4; OPN2; CSNBAD1
NCBI Protein Information
rhodopsin
UniProt Protein Name
Rhodopsin
UniProt Gene Name
RHO
UniProt Synonym Gene Names
OPN2
UniProt Entry Name
OPSD_HUMAN
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Product Notes
The RHO rho (Catalog #AAA52593) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rhodopsin / RHO Antibody (clone B630) IHC-plus reacts with Mouse, Bovine, Rat, Pig, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rhodopsin / RHO can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the RHO rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rhodopsin / RHO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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