ChIP (Chromatin Immunoprecipitation)
(Ring1B antibody (mAb) tested by ChIP-Seq. ChIP was performed using Ring1B antibody with 30 ug chromatin from the wild-type mouse ESC and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.)
Mouse Ring1B Monoclonal Antibody | anti-Ring1B antibody
Ring1B antibody (mAb)(Clone 3-3)
Gene Names
RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B
Reactivity
Human, Monkey, Mouse
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
Ring1B, Antibody; Ring1B antibody (mAb)(Clone 3-3); RNF2; BAP1; DING; BAP-1; HIPI3; RING2; anti-Ring1B antibody
Host
Mouse
Reactivity
Human, Monkey, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2021-03-03
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Ring1B antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This Ring1B antibody was raised against the recombinant full-length mouse protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
ChIP (Chromatin Immunoprecipitation)
(Ring1B antibody (mAb) tested by ChIP-Seq. ChIP was performed using Ring1B antibody with 30 ug chromatin from the wild-type mouse ESC and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.)
ChIP (Chromatin Immunoprecipitation)
(Ring1B antibody (mAb) tested by ChIP-Seq. ChIP was performed using Ring1B antibody with 30 ug chromatin from the wild-type mouse ESC and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19.5 million sequence tags were mapped to identify Ring1B binding sites. The image shows binding across a region of chromosome 2.)
ChIP (Chromatin Immunoprecipitation)
(Ring1B antibody (mAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 4.5 million human embryonic stem cells and 4 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.)
ChIP (Chromatin Immunoprecipitation)
(Ring1B antibody (mAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 4.5 million human embryonic stem cells and 4 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The two images show regions of strong binding on chromosomes 2 and 9.)
Related Product Information for anti-Ring1B antibody
Short Description: Our Ring1B antibody (mAb)(Clone 3-3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human, Monkey and Mouse samples.
Background: Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.
Background: Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.
Product Categories/Family for anti-Ring1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,258 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RING2
NCBI Official Synonym Full Names
ring finger protein 2
NCBI Official Symbol
RNF2
NCBI Official Synonym Symbols
BAP1; DING; BAP-1; HIPI3; RING2; RING1B
NCBI Protein Information
E3 ubiquitin-protein ligase RING2; HIP2-interacting protein 3; RING finger protein 1B; RING finger protein BAP-1; huntingtin-interacting protein 2-interacting protein 3; protein DinG
UniProt Protein Name
E3 ubiquitin-protein ligase RING2
UniProt Gene Name
RNF2
UniProt Synonym Gene Names
BAP1; DING; HIPI3; RING1B; HIP2-interacting protein 3; RING1b
UniProt Entry Name
RING2_HUMAN
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Product Notes
The Ring1B rnf2 (Catalog #AAA59811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ring1B antibody (mAb)(Clone 3-3) reacts with Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ring1B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Ring1B rnf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ring1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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