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Mouse RNF2 Monoclonal Antibody | anti-RNF2 antibody
RNF2 (RING Finger Protein 2, BAP-1, BAP1, DING, HIPI3, Ring1B, Ring2) (FITC)
Gene Names
RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B
Applications
Western Blot
Purity
Purified
Synonyms
RNF2, Antibody; RNF2 (RING Finger Protein 2, BAP-1, BAP1, DING, HIPI3, Ring1B, Ring2) (FITC); Ring Finger Protein 2; BAP-1; BAP1; DING; HIPI3; Ring1B; Ring2; anti-RNF2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B4
Specificity
Recognizes RNF2.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-RNF2 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
RNF2 (NP_009143, 164aa-223aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
IENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTSDDSGLELDNNNAAMAIDPVMDG
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in Raw 264.7.)
WB (Western Blot)
(RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in PC-12.)
WB (Western Blot)
(RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in NIH/3T3.)
Application Data
(Detection limit for recombinant GST tagged RNF2 is approximately 3ng/ml as a capture antibody.)
WB (Western Blot)
(RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in A-549 (Cat # L025V1).)
WB (Western Blot)
(RNF2 monoclonal antibody (M14), clone 2B4. Western Blot analysis of RNF2 expression in HepG2 (Cat # L019V1).)
Related Product Information for anti-RNF2 antibody
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq]
Product Categories/Family for anti-RNF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,258 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RING2
NCBI Official Synonym Full Names
ring finger protein 2
NCBI Official Symbol
RNF2
NCBI Official Synonym Symbols
BAP1; DING; BAP-1; HIPI3; RING2; RING1B
NCBI Protein Information
E3 ubiquitin-protein ligase RING2; HIP2-interacting protein 3; RING finger protein 1B; RING finger protein BAP-1; huntingtin-interacting protein 2-interacting protein 3; protein DinG
UniProt Protein Name
E3 ubiquitin-protein ligase RING2
UniProt Gene Name
RNF2
UniProt Synonym Gene Names
BAP1; DING; HIPI3; RING1B; HIP2-interacting protein 3; RING1b
UniProt Entry Name
RING2_HUMAN
