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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human ROR-gamma/RORC Monoclonal Antibody | anti-RORC antibody
ROR-gamma/RORC (RAR-related Orphan Receptor C)
Gene Names
RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
ROR-gamma/RORC, Antibody; ROR-gamma/RORC (RAR-related Orphan Receptor C); Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3 (NR1F3); RAR related orphan receptor C; Retinoid-related orphan receptor-gamma (RORG); Rorc; RZR GAMMA; RZRG; TOR; anti-RORC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
RORC/2941
Specificity
This MAb recognizes a protein of 63kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR and ROR are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Sequence Length
497
Applicable Applications for anti-RORC antibody
Flow Cytometry (FC), Immunohistochemistry (IHC) Paraffin
Application Notes
FC: 1-2ug/million cells (3% PFA-fixed cells)
IHC-P: 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
IHC-P: 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunogen
Recombinant full-length Human RORC protein
Cellular Localization
Nuclear
Hu-chromosome Location
1q21.3
Positive Control
MOLT4 cells. Human Lymphocytes. Liver or Skeletal muscle tissue.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941). Confirmation of Purity and Integrity of Antibody.)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed MOLT4 cells using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941); followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed MOLT4 cells using ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941); followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
IF (Immunofluorescence)
(Immunofluorescence staining of paraformaldehyde-fixed MOLT4 cells with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941); followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).)
IF (Immunofluorescence)
(Immunofluorescence staining of paraformaldehyde-fixed MOLT4 cells with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941); followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).)
IHC (Immunohistochemistry-Formalin)
(Formalin-fixed, paraffin-embedded human Kidney stained with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941).)
IHC (Immunohistochemistry-Formalin)
(Formalin-fixed, paraffin-embedded human Kidney stained with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941).)
IHC (Immunohistochemistry-Formalin)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941).)
IHC (Immunohistochemistry-Formalin)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ROR-gamma / RORC Mouse Monoclonal Antibody (RORC/2941).)
References
Hirose, T., et al. 1994. ROR: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle. Biochem. Biophys. Res. Comm. 205: 1976-1983.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63kDa
NCBI Official Full Name
nuclear receptor ROR-gamma isoform b
NCBI Official Synonym Full Names
RAR related orphan receptor C
NCBI Official Symbol
RORC
NCBI Official Synonym Symbols
TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
NCBI Protein Information
nuclear receptor ROR-gamma
UniProt Protein Name
Nuclear receptor ROR-gamma
UniProt Gene Name
RORC
UniProt Synonym Gene Names
NR1F3; RORG; RZRG
UniProt Entry Name
RORG_HUMAN
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Product Notes
The RORC rorc (Catalog #AAA23951) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ROR-gamma/RORC (RAR-related Orphan Receptor C) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ROR-gamma/RORC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry (IHC) Paraffin. FC: 1-2ug/million cells (3% PFA-fixed cells) IHC-P: 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the RORC rorc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROR-gamma/RORC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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