<
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)
Mouse RPA70 Monoclonal Antibody | anti-RPA70 antibody
RPA70 Mouse mAb
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human, Monkey, Mouse, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
RPA70, Antibody; RPA70 Mouse mAb; Dmrpa1; Drosophila Replication Protein A; DRPA; DSSB; HSSB; Human single stranded DNA binding protein; MST075; MSTP075; p70; REPA 1; REPA1; Replication factor A; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A 70kDa DNA binding subunit; Replication protein A1 (70kD); Replication protein A1 70kDa; Replication protein A1; RF A; RF-A protein 1; RFA; RFA1_HUMAN; RP A; RP-A p70; RPA 1; RPA 70; RPA; RPA1; Single stranded binding protein 70; Single stranded DNA binding protein; Single-stranded DNA-binding protein; Ssb70.; anti-RPA70 antibody
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RPA70 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human RPA70 protein fragments expressed in E coli.
Modification
Unmodified/Total
Function
It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.
PTM
Phosphorylated upon DNA damage, probably by ATM or ATR.
Belongs to the replication factor A protein 1 family.
Belongs to the replication factor A protein 1 family.
Subunit
Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
Preparation and Storage
Store at -20 degree C/1 year
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using RPA70 mouse mAb (dilution 1:100).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using RPA70 mouse mAb.)
WB (Western Blot)
(Western blot detection of RPA70 in HUVEC.Ha.MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of RPA70 in HUVEC.Ha.MCF7 and A549 cell lysates using RPA70 mouse mAb (1:2000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of RPA70 in Hela.A431.MCF7.COS7.HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of RPA70 in Hela.A431.MCF7.COS7.HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of RPA70 in Hela.293T.C6.3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
WB (Western Blot)
(Western blot detection of RPA70 in Hela.293T.C6.3T3 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Product Categories/Family for anti-RPA70 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
616
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1, 70kDa
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70; RP-A p70
UniProt Entry Name
RFA1_HUMAN
Similar Products
Product Notes
The RPA70 rpa1 (Catalog #AAA320372) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RPA70 Mouse mAb reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPA70 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RPA70 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.