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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue)
Mouse S100 Calcium Binding Protein A6 (S100A6) Monoclonal Antibody | anti-S100A6 antibody
Monoclonal Antibody to S100 Calcium Binding Protein A6 (S100A6)
Gene Names
S100A6; 2A9; PRA; 5B10; CABP; CACY; S10A6
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
S100 Calcium Binding Protein A6 (S100A6), Antibody; Monoclonal Antibody to S100 Calcium Binding Protein A6 (S100A6); anti-S100A6 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
1-1#
Specificity
The antibody is a mouse monoclonal antibody raised against S100A6. It has been selected for its ability to recognize S100A6 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL
Sequence Length
90
Applicable Applications for anti-S100A6 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant S100A6 (Met1~Gly90) expressed in E.coli.
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Prostate Gland Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue.)
WB (Western Blot)
(Western Blot: Sample: Recombinant S100A6, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant S100A6, Human.)
WB (Western Blot)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 3ug/ml Mouse Anti-Human S100A6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 3ug/ml Mouse Anti-Human S100A6 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human A549 Cells; Lane3: Human Mcf7 Cells.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human A549 Cells; Lane3: Human Mcf7 Cells.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,180 Da
NCBI Official Full Name
protein S100-A6
NCBI Official Synonym Full Names
S100 calcium binding protein A6
NCBI Official Symbol
S100A6
NCBI Official Synonym Symbols
2A9; PRA; 5B10; CABP; CACY; S10A6
NCBI Protein Information
protein S100-A6
UniProt Protein Name
Protein S100-A6
UniProt Gene Name
S100A6
UniProt Synonym Gene Names
CACY; PRA
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Product Notes
The S100A6 s100a6 (Catalog #AAA20295) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to S100 Calcium Binding Protein A6 (S100A6) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's S100 Calcium Binding Protein A6 (S100A6) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the S100A6 s100a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS - MACPLDQAIG LLVAIFHKYS GREGDKHTLS KKELKELIQK ELTIGSKLQD AEIARLMEDL. It is sometimes possible for the material contained within the vial of "S100 Calcium Binding Protein A6 (S100A6), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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