Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (CPTC-S100A4-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse S100A4 Monoclonal Antibody | anti-S100A4 antibody
S100A4 (Marker of Tumor Metastasis)
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
S100A4, Antibody; S100A4 (Marker of Tumor Metastasis); S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein; anti-S100A4 antibody
Host
Mouse
Reactivity
Human. Others not tested.
Clonality
Monoclonal
Isotype
IgG2c
Clone Number
CPTC-S100A4-3
Specificity
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100A4 has been implicated in the progression and prognosis of several forms of human cancer, e.g. breast, colorectal, gastric, pancreatic and bladder cancer, SCLC and oesophageal squamous cell carcinoma, among others. Poor prognosis associated with high S100A4 expression is accompanied by clear signs of disease progression, e.g. high histological and clinical grades and involvement of lymph nodes. Also indicative of poor prognosis is high S100A4 expression coupled with reduced E-cadherin expression in pancreatic, oral squamous cell carcinoma and in melanoma. S100A4 expression is inversely related with expression of metastasis suppressor nm23 and with prognosis of breast cancer. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
101
Applicable Applications for anti-S100A4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant human S100A4 full length protein
Positive Control
HeLa, A549 or A375 Cells. Placenta, small intestine.
Cellular Localization
Nucleus and cytoplasm.
Chromosome Location
1q21.3
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (CPTC-S100A4-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (CPTC-S100A4-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with S100A4 Mouse Monoclonal Antibody (CPTC-S100A4-3).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with S100A4 Mouse Monoclonal Antibody (CPTC-S100A4-3).)
References
Orre, L M, et al. Nature News, Nature Publishing Group, 10 June 201
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12kDa
NCBI Official Full Name
protein S100-A4
NCBI Official Synonym Full Names
S100 calcium binding protein A4
NCBI Official Symbol
S100A4
NCBI Official Synonym Symbols
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
NCBI Protein Information
protein S100-A4
UniProt Protein Name
Protein S100-A4
UniProt Gene Name
S100A4
UniProt Synonym Gene Names
CAPL; MTS1
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Product Notes
The S100A4 s100a4 (Catalog #AAA214812) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The S100A4 (Marker of Tumor Metastasis) reacts with Human. Others not tested. and may cross-react with other species as described in the data sheet. AAA Biotech's S100A4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the S100A4 s100a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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