ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).)
Mouse anti-Human SAFB1 Monoclonal Antibody | anti-SAFB antibody
SAFB1 Antibody
Gene Names
SAFB; HAP; HET; SAFB1; SAF-B1
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Synonyms
SAFB1, Antibody; SAFB1 Antibody; Purified Mouse Monoclonal Antibody (Mab); DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B1; SAFB 1; SAFB; SAFB1; SAFB1_HUMAN; scaffold attachment factor B1.; anti-SAFB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Sequence Length
917
Applicable Applications for anti-SAFB antibody
ICC (Immunocytochemistry), WB (Western Blot)
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200).)
WB (Western Blot)
(Western blot detection of SAFB1 in HelaNE,A431,MDA-MB-468,Breast cancer,ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.)
WB (Western Blot)
(Western blot detection of SAFB1 in HelaNE,A431,MDA-MB-468,Breast cancer,ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.)
Related Product Information for anti-SAFB antibody
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.
References
Oesterreich S.,et al.J. Cell. Biochem. 67:275-286(1997). Ota T.,et al.Nat. Genet. 36:40-45(2004). Grimwood J.,et al.Nature 428:529-535(2004). Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. Bienvenut W.V.,et al.Submitted (JUL-2009) to UniProtKB.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102642
NCBI Official Full Name
scaffold attachment factor B1 isoform 1
NCBI Official Synonym Full Names
scaffold attachment factor B
NCBI Official Symbol
SAFB
NCBI Official Synonym Symbols
HAP; HET; SAFB1; SAF-B1
NCBI Protein Information
scaffold attachment factor B1
UniProt Protein Name
Scaffold attachment factor B1
UniProt Gene Name
SAFB
UniProt Synonym Gene Names
HAP; HET; SAFB1; SAF-B; SAF-B1; HSP27 ERE-TATA-binding protein
UniProt Entry Name
SAFB1_HUMAN
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Product Notes
The SAFB safb (Catalog #AAA290524) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SAFB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAFB1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SAFB safb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAFB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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